Projets par an
Résumé
most important processes that takes place in living cells. Human phosphoserine
phosphatase (hPSP) is a key enzyme in the production of serine by the
dephosphorylation of phospho-l-serine. It is directly involved in the biosynthesis
of other important metabolites such as glycine and d-serine (a
neuromodulator). hPSP is involved in the survival mechanism of cancer cells
and has recently been found to be an essential biomarker. Here, three new highresolution crystal structures of hPSP (1.5–2.0 Ang.) in complexes with phosphoserine and with serine, which are the substrate and the product of the reaction, respectively, and in complex with a noncleavable substrate analogue (homocysteic acid) are presented. New types of interactions take place between the
enzyme and its ligands. Moreover, the loop involved in the open/closed state of
the enzyme is fully refined in a totally unfolded conformation. This loop is
further studied through molecular-dynamics simulations. Finally, all of these
analyses allow a more complete reaction mechanism for this enzyme to be
proposed which is consistent with previous publications on the subject.
langue originale | Anglais |
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Pages (de - à) | 592-604 |
Nombre de pages | 13 |
journal | Acta crystallographica. Section D: Structural Biology |
Volume | 75 |
Les DOIs | |
Etat de la publication | Publié - 1 juin 2019 |
Empreinte digitale
Examiner les sujets de recherche de « Crystal structures and snapshots along the reaction pathway of human phosphoserine phosphatase ». Ensemble, ils forment une empreinte digitale unique.Projets
- 2 Terminé
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CÉCI – Consortium des Équipements de Calcul Intensif
CHAMPAGNE, B. (Responsable du Projet), Lazzaroni, R. (Responsable du Projet), Geuzaine , C. (Co-investigateur), Chatelain, P. (Co-investigateur) & Knaepen, B. (Co-investigateur)
1/01/18 → 31/12/22
Projet: Recherche
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Structural and inhibition study of SerB2 (Mycobacterium tuberculosis phosphoserine phosphatase)
Haufroid, M. (Chercheur) & Wouters, J. (Promoteur)
1/09/16 → 31/08/22
Projet: Projet de thèse
Équipement
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Physico-chimie et caractérisation (PC2)
Wouters, J. (!!Manager), Aprile, C. (!!Manager) & Fusaro, L. (!!Manager)
Plateforme technologique Caracterisation physico-chimiquesEquipement/installations: Plateforme technolgique
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Plateforme Technologique Calcul Intensif
Champagne, B. (!!Manager)
Plateforme technologique Calcul intensifEquipement/installations: Plateforme technolgique
Ensembles de données
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Human phosphoserine phosphatase with serine and phosphate
Wouters, J. (Créateur), Haufroid, M. (Créateur) & MIRGAUX, M. (Créateur), University of Namur, 12 juin 2019
DOI: 10.2210/pdb6HYY/pdb
Ensemble de données
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Human phosphoserine phosphatase with substrate analogue homo-cysteic acid
Wouters, J. (Créateur), Haufroid, M. (Créateur) & MIRGAUX, M. (Créateur), University of Namur, 12 juin 2019
DOI: 10.2210/pdb6Q6J/pdb
Ensemble de données
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PSPH Human phosphoserine phosphatase
Wouters, J. (Créateur), Haufroid, M. (Créateur) & MIRGAUX, M. (Créateur), University of Namur, 12 juin 2019
DOI: 10.2210/pdb6HYJ/pdb
Ensemble de données