Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes

Stéphane Demine; Silvia Veronica Tejerina Vargas; Benoît Bihin; Marc Thiry; Reddy Nagabushana; Patricia Renard; Martine Raes; Michel Jadot; Thierry Arnould

doi:10.1002/jcp.25994

Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes

Stéphane Demine, Silvia Veronica Tejerina Vargas, Benoît Bihin, Marc Thiry, Reddy Nagabushana, Patricia Renard, Martine Raes, Michel Jadot, Thierry Arnould

Résultats de recherche: Contribution à un journal/une revue › Article

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Résumé

Obesity is characterized by an excessive triacylglycerol accumulation in white adipocytes. Various mechanisms allowing the tight regulation of triacylglycerol storage and mobilization by lipid droplet-associated proteins as well as lipolytic enzymes have been identified. Increasing energy expenditure by inducing a mild uncoupling of mitochondria in adipocytes might represent a putative interesting anti-obesity strategy as it reduces the adipose tissue triacylglycerol content (limiting alterations caused by cell hypertrophy) by stimulating lipolysis through yet unknown mechanisms, limiting the adverse effects of adipocyte hypertrophy. Herein, the molecular mechanisms involved in lipolysis induced by a mild uncoupling of mitochondria in white 3T3-L1 adipocytes were characterized. Mitochondrial uncoupling-induced lipolysis was found to be independent from canonical pathways that involve lipolytic enzymes such as HSL and ATGL. Finally, enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles. This new mechanism of triacylglycerol breakdown in adipocytes exposed to mild uncoupling provides new insights on the biology of adipocytes dealing with mitochondria forced to dissipate energy.

langue originale	Anglais
Pages (de - à)	1247-1265
Nombre de pages	18
journal	Journal of Cellular Physiology
Volume	233
Numéro de publication	2
Les DOIs	https://doi.org/10.1002/jcp.25994
état	Publié - 1 févr. 2018

Empreinte digitale

Lipolysis

Autophagy

Adipocytes

Mitochondria

Triglycerides

Hypertrophy

Lipids

Obesity

White Adipocytes

Enzymes

Energy Metabolism

Tissue

Adipose Tissue

Proteins

Citer ceci

Demine, S., Tejerina Vargas, S. V., Bihin, B., Thiry, M., Nagabushana, R., Renard, P., ... Arnould, T. (2018). Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes. Journal of Cellular Physiology, 233(2), 1247-1265. https://doi.org/10.1002/jcp.25994

Demine, Stéphane ; Tejerina Vargas, Silvia Veronica ; Bihin, Benoît ; Thiry, Marc ; Nagabushana, Reddy ; Renard, Patricia ; Raes, Martine ; Jadot, Michel ; Arnould, Thierry. / Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes. Dans: Journal of Cellular Physiology. 2018 ; Vol 233, Numéro 2. p. 1247-1265.

@article{70f1b4f099034491afc5f1c4ba8fcd14,

title = "Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes",

abstract = "Obesity is characterized by an excessive triacylglycerol accumulation in white adipocytes. Various mechanisms allowing the tight regulation of triacylglycerol storage and mobilization by lipid droplet-associated proteins as well as lipolytic enzymes have been identified. Increasing energy expenditure by inducing a mild uncoupling of mitochondria in adipocytes might represent a putative interesting anti-obesity strategy as it reduces the adipose tissue triacylglycerol content (limiting alterations caused by cell hypertrophy) by stimulating lipolysis through yet unknown mechanisms, limiting the adverse effects of adipocyte hypertrophy. Herein, the molecular mechanisms involved in lipolysis induced by a mild uncoupling of mitochondria in white 3T3-L1 adipocytes were characterized. Mitochondrial uncoupling-induced lipolysis was found to be independent from canonical pathways that involve lipolytic enzymes such as HSL and ATGL. Finally, enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles. This new mechanism of triacylglycerol breakdown in adipocytes exposed to mild uncoupling provides new insights on the biology of adipocytes dealing with mitochondria forced to dissipate energy.",

keywords = "mitochondrial uncoupling; lipolysis; autophagy; HSL; ATGL; adipocytes; lipid metabolism; glycerol, HSL, autophagy, ATGL, lipid metabolism, mitochondrial uncoupling, lipolysis, adipocytes, glycerol",

author = "St{\'e}phane Demine and {Tejerina Vargas}, {Silvia Veronica} and Beno{\^i}t Bihin and Marc Thiry and Reddy Nagabushana and Patricia Renard and Martine Raes and Michel Jadot and Thierry Arnould",

year = "2018",

month = "2",

day = "1",

doi = "10.1002/jcp.25994",

language = "English",

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Demine, S, Tejerina Vargas, SV, Bihin, B, Thiry, M, Nagabushana, R , Renard, P , Raes, M , Jadot, M & Arnould, T 2018, 'Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes', Journal of Cellular Physiology, VOL. 233, Numéro 2, p. 1247-1265. https://doi.org/10.1002/jcp.25994

Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes. / Demine, Stéphane; Tejerina Vargas, Silvia Veronica; Bihin, Benoît; Thiry, Marc; Nagabushana, Reddy ; Renard, Patricia ; Raes, Martine ; Jadot, Michel ; Arnould, Thierry.

Dans: Journal of Cellular Physiology, Vol 233, Numéro 2, 01.02.2018, p. 1247-1265.

Résultats de recherche: Contribution à un journal/une revue › Article

TY - JOUR

T1 - Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes

AU - Demine, Stéphane

AU - Tejerina Vargas, Silvia Veronica

AU - Bihin, Benoît

AU - Thiry, Marc

AU - Nagabushana, Reddy

AU - Renard, Patricia

AU - Raes, Martine

AU - Jadot, Michel

AU - Arnould, Thierry

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Obesity is characterized by an excessive triacylglycerol accumulation in white adipocytes. Various mechanisms allowing the tight regulation of triacylglycerol storage and mobilization by lipid droplet-associated proteins as well as lipolytic enzymes have been identified. Increasing energy expenditure by inducing a mild uncoupling of mitochondria in adipocytes might represent a putative interesting anti-obesity strategy as it reduces the adipose tissue triacylglycerol content (limiting alterations caused by cell hypertrophy) by stimulating lipolysis through yet unknown mechanisms, limiting the adverse effects of adipocyte hypertrophy. Herein, the molecular mechanisms involved in lipolysis induced by a mild uncoupling of mitochondria in white 3T3-L1 adipocytes were characterized. Mitochondrial uncoupling-induced lipolysis was found to be independent from canonical pathways that involve lipolytic enzymes such as HSL and ATGL. Finally, enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles. This new mechanism of triacylglycerol breakdown in adipocytes exposed to mild uncoupling provides new insights on the biology of adipocytes dealing with mitochondria forced to dissipate energy.

AB - Obesity is characterized by an excessive triacylglycerol accumulation in white adipocytes. Various mechanisms allowing the tight regulation of triacylglycerol storage and mobilization by lipid droplet-associated proteins as well as lipolytic enzymes have been identified. Increasing energy expenditure by inducing a mild uncoupling of mitochondria in adipocytes might represent a putative interesting anti-obesity strategy as it reduces the adipose tissue triacylglycerol content (limiting alterations caused by cell hypertrophy) by stimulating lipolysis through yet unknown mechanisms, limiting the adverse effects of adipocyte hypertrophy. Herein, the molecular mechanisms involved in lipolysis induced by a mild uncoupling of mitochondria in white 3T3-L1 adipocytes were characterized. Mitochondrial uncoupling-induced lipolysis was found to be independent from canonical pathways that involve lipolytic enzymes such as HSL and ATGL. Finally, enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles. This new mechanism of triacylglycerol breakdown in adipocytes exposed to mild uncoupling provides new insights on the biology of adipocytes dealing with mitochondria forced to dissipate energy.

KW - mitochondrial uncoupling; lipolysis; autophagy; HSL; ATGL; adipocytes; lipid metabolism; glycerol

KW - HSL

KW - autophagy

KW - ATGL

KW - lipid metabolism

KW - mitochondrial uncoupling

KW - lipolysis

KW - adipocytes

KW - glycerol

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U2 - 10.1002/jcp.25994

DO - 10.1002/jcp.25994