Projets par an
Résumé
Obesity is characterized by an excessive triacylglycerol accumulation in white adipocytes. Various mechanisms allowing the tight regulation of triacylglycerol storage and mobilization by lipid droplet-associated proteins as well as lipolytic enzymes have been identified. Increasing energy expenditure by inducing a mild uncoupling of mitochondria in adipocytes might represent a putative interesting anti-obesity strategy as it reduces the adipose tissue triacylglycerol content (limiting alterations caused by cell hypertrophy) by stimulating lipolysis through yet unknown mechanisms, limiting the adverse effects of adipocyte hypertrophy. Herein, the molecular mechanisms involved in lipolysis induced by a mild uncoupling of mitochondria in white 3T3-L1 adipocytes were characterized. Mitochondrial uncoupling-induced lipolysis was found to be independent from canonical pathways that involve lipolytic enzymes such as HSL and ATGL. Finally, enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles. This new mechanism of triacylglycerol breakdown in adipocytes exposed to mild uncoupling provides new insights on the biology of adipocytes dealing with mitochondria forced to dissipate energy.
langue originale | Anglais |
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Pages (de - à) | 1247-1265 |
Nombre de pages | 18 |
journal | Journal of Cellular Physiology |
Volume | 233 |
Numéro de publication | 2 |
Les DOIs | |
Etat de la publication | Publié - 1 févr. 2018 |
Empreinte digitale
Examiner les sujets de recherche de « Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes ». Ensemble, ils forment une empreinte digitale unique.Projets
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Effets du découplage mitochondrial dans les adipocytes sur la libération de molécules de communication intercellulaire comme les acides gras et les adipokines: recherche des mécanismes de la lipolyse et caractérisation de sécrétomes
Arnould, T. (Co-investigateur), DEMINE, S. (Chercheur) & Jadot, M. (Co-investigateur)
1/10/10 → 30/09/14
Projet: Projet de thèse
Équipement
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Morphologie - Imagerie
Cecchet, F. (!!Manager) & Renard, H.-F. (!!Manager)
Plateforme technologique Morphologie, imagerieEquipement/installations: Plateforme technolgique
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Physico-chimie et caractérisation (PC2)
Wouters, J. (!!Manager), Aprile, C. (!!Manager) & Fusaro, L. (!!Manager)
Plateforme technologique Caracterisation physico-chimiquesEquipement/installations: Plateforme technolgique
Thèses de l'étudiant
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Effects of a mitochondrial uncoupling on lipolysis and adipokine expression in 3T3-L1 murine adipocytes
Demine, S. (Auteur)Arnould, T. (Promoteur), Jadot, M. (Copromoteur), De Bolle, X. (Président), Raes, M. (Jury), KEIJER, J. (Jury) & Bertrand, L. (Jury), 26 avr. 2016Student thesis: Doc types › Docteur en Sciences