Résumé
| langue | Anglais |
|---|---|
| Pages | 1247-1265 |
| Nombre de pages | 18 |
| journal | Journal of Cellular Physiology |
| Volume | 233 |
| Les DOIs | |
| état | Publié - 1 févr. 2018 |
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Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes. / Demine, Stéphane; Tejerina Vargas, Silvia Veronica; Bihin, Benoît; Thiry, Marc; Nagabushana, Reddy; Renard, Patricia; Raes, Martine; Jadot, Michel; Arnould, Thierry.
Dans: Journal of Cellular Physiology, Vol 233, 01.02.2018, p. 1247-1265.Résultats de recherche: Contribution à un journal/une revue › Article
TY - JOUR
T1 - Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes
AU - Demine,Stéphane
AU - Tejerina Vargas,Silvia Veronica
AU - Bihin,Benoît
AU - Thiry,Marc
AU - Nagabushana,Reddy
AU - Renard,Patricia
AU - Raes,Martine
AU - Jadot,Michel
AU - Arnould,Thierry
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Obesity is characterized by an excessive triacylglycerol accumulation in white adipocytes. Various mechanisms allowing the tight regulation of triacylglycerol storage and mobilization by lipid droplet-associated proteins as well as lipolytic enzymes have been identified. Increasing energy expenditure by inducing a mild uncoupling of mitochondria in adipocytes might represent a putative interesting anti-obesity strategy as it reduces the adipose tissue triacylglycerol content (limiting alterations caused by cell hypertrophy) by stimulating lipolysis through yet unknown mechanisms, limiting the adverse effects of adipocyte hypertrophy. Herein, the molecular mechanisms involved in lipolysis induced by a mild uncoupling of mitochondria in white 3T3-L1 adipocytes were characterized. Mitochondrial uncoupling-induced lipolysis was found to be independent from canonical pathways that involve lipolytic enzymes such as HSL and ATGL. Finally, enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles. This new mechanism of triacylglycerol breakdown in adipocytes exposed to mild uncoupling provides new insights on the biology of adipocytes dealing with mitochondria forced to dissipate energy.
AB - Obesity is characterized by an excessive triacylglycerol accumulation in white adipocytes. Various mechanisms allowing the tight regulation of triacylglycerol storage and mobilization by lipid droplet-associated proteins as well as lipolytic enzymes have been identified. Increasing energy expenditure by inducing a mild uncoupling of mitochondria in adipocytes might represent a putative interesting anti-obesity strategy as it reduces the adipose tissue triacylglycerol content (limiting alterations caused by cell hypertrophy) by stimulating lipolysis through yet unknown mechanisms, limiting the adverse effects of adipocyte hypertrophy. Herein, the molecular mechanisms involved in lipolysis induced by a mild uncoupling of mitochondria in white 3T3-L1 adipocytes were characterized. Mitochondrial uncoupling-induced lipolysis was found to be independent from canonical pathways that involve lipolytic enzymes such as HSL and ATGL. Finally, enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles. This new mechanism of triacylglycerol breakdown in adipocytes exposed to mild uncoupling provides new insights on the biology of adipocytes dealing with mitochondria forced to dissipate energy.
KW - mitochondrial uncoupling; lipolysis; autophagy; HSL; ATGL; adipocytes; lipid metabolism; glycerol
U2 - 10.1002/jcp.25994
DO - 10.1002/jcp.25994
M3 - Article
VL - 233
SP - 1247
EP - 1265
JO - Journal of Cellular Physiology
T2 - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
ER -