Head-on immobilization of DNA fragments on CVD-diamond layers

S. Wenmackers, P. Christiaens, W. Deferme, M. Daenen, K. Haenen, M. Nesládek, P. Wagner, V. Vermeeren, L. Michiels, M. VandeVen, M. Ameloot, J. Wouters, L. Naelaerts, Z. Mekhalif

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Résumé

Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.

langue originaleAnglais
titreMaterials Science Forum
Pages267-272
Nombre de pages6
Volume492-493
étatPublié - 1 déc. 2005
Evénement8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004 - Leuven, Belgique
Durée: 11 juil. 200414 juil. 2004

Série de publications

NomMaterials Science Forum
Volume492-493
ISSN (imprimé)02555476

Une conférence

Une conférence8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004
PaysBelgique
La villeLeuven
période11/07/0414/07/04

    Empreinte digitale

Contient cette citation

Wenmackers, S., Christiaens, P., Deferme, W., Daenen, M., Haenen, K., Nesládek, M., ... Mekhalif, Z. (2005). Head-on immobilization of DNA fragments on CVD-diamond layers. Dans Materials Science Forum (Vol 492-493, p. 267-272). (Materials Science Forum; Vol 492-493).