Head-on immobilization of DNA fragments on CVD-diamond layers

S. Wenmackers; P. Christiaens; W. Deferme; M. Daenen; K. Haenen; M. Nesládek; P. Wagner; V. Vermeeren; L. Michiels; M. VandeVen; M. Ameloot; J. Wouters; L. Naelaerts; Z. Mekhalif

Head-on immobilization of DNA fragments on CVD-diamond layers

S. Wenmackers, P. Christiaens, W. Deferme, M. Daenen, K. Haenen, M. Nesládek, P. Wagner, V. Vermeeren, L. Michiels, M. VandeVen, M. Ameloot, J. Wouters, L. Naelaerts, Z. Mekhalif

Research output: Contribution in Book/Catalog/Report/Conference proceeding › Conference contribution

Abstract

Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.

Original language	English
Title of host publication	Materials Science Forum
Pages	267-272
Number of pages	6
Volume	492-493
Publication status	Published - 1 Dec 2005
Event	8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004 - Leuven, Belgium Duration: 11 Jul 2004 → 14 Jul 2004

Publication series

Name	Materials Science Forum
Volume	492-493
ISSN (Print)	02555476

Conference

Conference	8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004
Country/Territory	Belgium
City	Leuven
Period	11/07/04 → 14/07/04

Keywords

Affinity biosensors
DNA hybridisation
Synthetic diamond layers
X-ray photoemission spectroscopy

Cite this

@inproceedings{4418b4790a484b598d6b1c55108e1a63,

title = "Head-on immobilization of DNA fragments on CVD-diamond layers",

abstract = "Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.",

keywords = "Affinity biosensors, DNA hybridisation, Synthetic diamond layers, X-ray photoemission spectroscopy",

author = "S. Wenmackers and P. Christiaens and W. Deferme and M. Daenen and K. Haenen and M. Nesl{\'a}dek and P. Wagner and V. Vermeeren and L. Michiels and M. VandeVen and M. Ameloot and J. Wouters and L. Naelaerts and Z. Mekhalif",

year = "2005",

month = dec,

day = "1",

language = "English",

isbn = "0878499709",

volume = "492-493",

series = "Materials Science Forum",

pages = "267--272",

booktitle = "Materials Science Forum",

note = "8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004 ; Conference date: 11-07-2004 Through 14-07-2004",

}

Wenmackers, S, Christiaens, P, Deferme, W, Daenen, M, Haenen, K, Nesládek, M, Wagner, P, Vermeeren, V, Michiels, L, VandeVen, M, Ameloot, M, Wouters, J, Naelaerts, L & Mekhalif, Z 2005, Head-on immobilization of DNA fragments on CVD-diamond layers. in Materials Science Forum. vol. 492-493, Materials Science Forum, vol. 492-493, pp. 267-272, 8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004, Leuven, Belgium, 11/07/04.

TY - GEN

T1 - Head-on immobilization of DNA fragments on CVD-diamond layers

AU - Wenmackers, S.

AU - Christiaens, P.

AU - Deferme, W.

AU - Daenen, M.

AU - Haenen, K.

AU - Nesládek, M.

AU - Wagner, P.

AU - Vermeeren, V.

AU - Michiels, L.

AU - VandeVen, M.

AU - Ameloot, M.

AU - Wouters, J.

AU - Naelaerts, L.

AU - Mekhalif, Z.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.

AB - Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.

KW - Affinity biosensors

KW - DNA hybridisation

KW - Synthetic diamond layers

KW - X-ray photoemission spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=35148824400&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:35148824400

SN - 0878499709

SN - 9780878499700

VL - 492-493

T3 - Materials Science Forum

SP - 267

EP - 272

BT - Materials Science Forum

T2 - 8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004

Y2 - 11 July 2004 through 14 July 2004

ER -

Head-on immobilization of DNA fragments on CVD-diamond layers

Abstract

Publication series

Conference

Keywords

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Fingerprint

Synthesis, Irradiation and Analysis of Materials (SIAM)

Cite this

Head-on immobilization of DNA fragments on CVD-diamond layers

Abstract

Publication series

Conference

Keywords

Other files and links

Fingerprint

Equipment

Synthesis, Irradiation and Analysis of Materials (SIAM)

Cite this