Head-on immobilization of DNA fragments on CVD-diamond layers

S. Wenmackers, P. Christiaens, W. Deferme, M. Daenen, K. Haenen, M. Nesládek, P. Wagner, V. Vermeeren, L. Michiels, M. VandeVen, M. Ameloot, J. Wouters, L. Naelaerts, Z. Mekhalif

Research output: Contribution in Book/Catalog/Report/Conference proceedingConference contribution

Abstract

Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.

Original languageEnglish
Title of host publicationMaterials Science Forum
Pages267-272
Number of pages6
Volume492-493
Publication statusPublished - 1 Dec 2005
Event8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004 - Leuven, Belgium
Duration: 11 Jul 200414 Jul 2004

Publication series

NameMaterials Science Forum
Volume492-493
ISSN (Print)02555476

Conference

Conference8th International Symposium on Multifunctional and Functionally Graded Materials, FGM2004
CountryBelgium
CityLeuven
Period11/07/0414/07/04

Keywords

  • Affinity biosensors
  • DNA hybridisation
  • Synthetic diamond layers
  • X-ray photoemission spectroscopy

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    Wenmackers, S., Christiaens, P., Deferme, W., Daenen, M., Haenen, K., Nesládek, M., Wagner, P., Vermeeren, V., Michiels, L., VandeVen, M., Ameloot, M., Wouters, J., Naelaerts, L., & Mekhalif, Z. (2005). Head-on immobilization of DNA fragments on CVD-diamond layers. In Materials Science Forum (Vol. 492-493, pp. 267-272). (Materials Science Forum; Vol. 492-493).