UVB-induced premature senescence of human diploid skin fibroblasts

Florence Chainiaux, Joao Pedro Magalhaes, François Eliaers, José Remacle, Olivier Toussaint

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

In this work, we show that repeated stresses with UVB (290-320nm) induce stress-induced premature senescence (SIPS) of skin human diploid fibroblasts (HDFs). HDFs at early cumulative population doublings were exposed three or five times to increasing subcytotoxic doses of UVB with one stress per day. After 2 days of recovery, several biomarkers of replicative senescence were established. First, there was an increase in the proportion of cells positive for senescence-associated β-galactosidase activity. Second, there was a loss of replicative potential as assessed by a very low level of [3H]-thymidine incorporation. Third, the steady-state level of the mRNA of three senescence-associated genes, i.e. fibronectin, osteonectin and SM22, was increased in HDFs at 72h after three and five exposures to UVB. In conclusion, these results suggest that it is possible to induce SIPS in HDFs after repeated exposures to subcytotoxic doses of UVB. This model could be used to test whether HDFs in UVB-induced premature senescence are able to promote epithelial cell growth and tumorigenesis in skin, as shown recently with HDFs in H2O2-induced premature senescence.

langue originaleAnglais
Pages (de - à)1331-1339
Nombre de pages9
journalThe international journal of Biochemistry & Cell biology
Volume34
Numéro de publication11
Les DOIs
étatPublié - 1 nov. 2002

Empreinte digitale

Fibroblasts
Diploidy
Skin
Cell Aging
Osteonectin
Galactosidases
Cell growth
Biomarkers
Fibronectins
Thymidine
Carcinogenesis
Genes
Epithelial Cells
Recovery
Messenger RNA
Growth
Population

Citer ceci

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title = "UVB-induced premature senescence of human diploid skin fibroblasts",
abstract = "In this work, we show that repeated stresses with UVB (290-320nm) induce stress-induced premature senescence (SIPS) of skin human diploid fibroblasts (HDFs). HDFs at early cumulative population doublings were exposed three or five times to increasing subcytotoxic doses of UVB with one stress per day. After 2 days of recovery, several biomarkers of replicative senescence were established. First, there was an increase in the proportion of cells positive for senescence-associated β-galactosidase activity. Second, there was a loss of replicative potential as assessed by a very low level of [3H]-thymidine incorporation. Third, the steady-state level of the mRNA of three senescence-associated genes, i.e. fibronectin, osteonectin and SM22, was increased in HDFs at 72h after three and five exposures to UVB. In conclusion, these results suggest that it is possible to induce SIPS in HDFs after repeated exposures to subcytotoxic doses of UVB. This model could be used to test whether HDFs in UVB-induced premature senescence are able to promote epithelial cell growth and tumorigenesis in skin, as shown recently with HDFs in H2O2-induced premature senescence.",
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UVB-induced premature senescence of human diploid skin fibroblasts. / Chainiaux, Florence; Magalhaes, Joao Pedro; Eliaers, François; Remacle, José; Toussaint, Olivier.

Dans: The international journal of Biochemistry & Cell biology, Vol 34, Numéro 11, 01.11.2002, p. 1331-1339.

Résultats de recherche: Contribution à un journal/une revueArticle

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AU - Magalhaes, Joao Pedro

AU - Eliaers, François

AU - Remacle, José

AU - Toussaint, Olivier

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