TY - JOUR
T1 - UVB-induced premature senescence of human diploid skin fibroblasts
AU - Chainiaux, Florence
AU - Magalhaes, Joao Pedro
AU - Eliaers, François
AU - Remacle, José
AU - Toussaint, Olivier
PY - 2002/11/1
Y1 - 2002/11/1
N2 - In this work, we show that repeated stresses with UVB (290-320nm) induce stress-induced premature senescence (SIPS) of skin human diploid fibroblasts (HDFs). HDFs at early cumulative population doublings were exposed three or five times to increasing subcytotoxic doses of UVB with one stress per day. After 2 days of recovery, several biomarkers of replicative senescence were established. First, there was an increase in the proportion of cells positive for senescence-associated β-galactosidase activity. Second, there was a loss of replicative potential as assessed by a very low level of [3H]-thymidine incorporation. Third, the steady-state level of the mRNA of three senescence-associated genes, i.e. fibronectin, osteonectin and SM22, was increased in HDFs at 72h after three and five exposures to UVB. In conclusion, these results suggest that it is possible to induce SIPS in HDFs after repeated exposures to subcytotoxic doses of UVB. This model could be used to test whether HDFs in UVB-induced premature senescence are able to promote epithelial cell growth and tumorigenesis in skin, as shown recently with HDFs in H2O2-induced premature senescence.
AB - In this work, we show that repeated stresses with UVB (290-320nm) induce stress-induced premature senescence (SIPS) of skin human diploid fibroblasts (HDFs). HDFs at early cumulative population doublings were exposed three or five times to increasing subcytotoxic doses of UVB with one stress per day. After 2 days of recovery, several biomarkers of replicative senescence were established. First, there was an increase in the proportion of cells positive for senescence-associated β-galactosidase activity. Second, there was a loss of replicative potential as assessed by a very low level of [3H]-thymidine incorporation. Third, the steady-state level of the mRNA of three senescence-associated genes, i.e. fibronectin, osteonectin and SM22, was increased in HDFs at 72h after three and five exposures to UVB. In conclusion, these results suggest that it is possible to induce SIPS in HDFs after repeated exposures to subcytotoxic doses of UVB. This model could be used to test whether HDFs in UVB-induced premature senescence are able to promote epithelial cell growth and tumorigenesis in skin, as shown recently with HDFs in H2O2-induced premature senescence.
KW - Fibroblasts
KW - Stress-induced premature senescence
KW - Tumorigenesis
KW - UVB
UR - http://www.scopus.com/inward/record.url?scp=0036828681&partnerID=8YFLogxK
U2 - 10.1016/S1357-2725(02)00022-5
DO - 10.1016/S1357-2725(02)00022-5
M3 - Article
C2 - 12200029
AN - SCOPUS:0036828681
SN - 1357-2725
VL - 34
SP - 1331
EP - 1339
JO - The international journal of Biochemistry & Cell biology
JF - The international journal of Biochemistry & Cell biology
IS - 11
ER -