UVB-induced premature senescence of human diploid skin fibroblasts

Florence Chainiaux, Joao Pedro Magalhaes, François Eliaers, José Remacle, Olivier Toussaint

Research output: Contribution to journalArticle

Abstract

In this work, we show that repeated stresses with UVB (290-320nm) induce stress-induced premature senescence (SIPS) of skin human diploid fibroblasts (HDFs). HDFs at early cumulative population doublings were exposed three or five times to increasing subcytotoxic doses of UVB with one stress per day. After 2 days of recovery, several biomarkers of replicative senescence were established. First, there was an increase in the proportion of cells positive for senescence-associated β-galactosidase activity. Second, there was a loss of replicative potential as assessed by a very low level of [3H]-thymidine incorporation. Third, the steady-state level of the mRNA of three senescence-associated genes, i.e. fibronectin, osteonectin and SM22, was increased in HDFs at 72h after three and five exposures to UVB. In conclusion, these results suggest that it is possible to induce SIPS in HDFs after repeated exposures to subcytotoxic doses of UVB. This model could be used to test whether HDFs in UVB-induced premature senescence are able to promote epithelial cell growth and tumorigenesis in skin, as shown recently with HDFs in H2O2-induced premature senescence.

Original languageEnglish
Pages (from-to)1331-1339
Number of pages9
JournalThe international journal of Biochemistry & Cell biology
Volume34
Issue number11
DOIs
Publication statusPublished - 1 Nov 2002

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Keywords

  • Fibroblasts
  • Stress-induced premature senescence
  • Tumorigenesis
  • UVB

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