TY - JOUR
T1 - Proteomics analysis of the endogenous, constitutive, leaf SUMOylome
AU - Colignon, Bertrand
AU - Delaive, Edouard
AU - Dieu, Marc
AU - Demazy, Catherine
AU - Muhovski, Yordan
AU - Wallon, Cindy
AU - Raes, Martine
AU - Mauro, Sergio
N1 - Funding Information:
Bertrand Colignon was supported by a fellowship of the FRIA (Brussels, BE). The MS MaSUN platform is supported by the FRS-FNRS (Brussels, BE) and the Université de Namur, Belgique. The authors thank Gilles San Martin for the statistical analyses.
Publisher Copyright:
© 2016
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/1/6
Y1 - 2017/1/6
N2 - SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined. Significance The authors have no conflicts of interest to declare. All authors have approved the manuscript and agree with submission to Journal of Proteomics.
AB - SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined. Significance The authors have no conflicts of interest to declare. All authors have approved the manuscript and agree with submission to Journal of Proteomics.
KW - 3D gel electrophoresis
KW - Endogenous SUMO conjugates
KW - Post translational modification
KW - Solanum tuberosum
KW - SUMOylation
UR - http://www.scopus.com/inward/record.url?scp=84990943412&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2016.09.012
DO - 10.1016/j.jprot.2016.09.012
M3 - Article
C2 - 27671789
SN - 1874-3919
VL - 150
SP - 268
EP - 280
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -