Proteomics analysis of the endogenous, constitutive, leaf SUMOylome

Bertrand Colignon, Edouard Delaive, Marc Dieu, Catherine Demazy, Yordan Muhovski, Cindy Wallon, Martine Raes, Sergio Mauro

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined. Significance The authors have no conflicts of interest to declare. All authors have approved the manuscript and agree with submission to Journal of Proteomics.

langueAnglais
Pages268-280
Nombre de pages13
journalJournal of Proteomics
Volume150
Les DOIs
étatPublié - 6 janv. 2017

Empreinte digitale

Sumoylation
Proteomics
Gels
Solanum tuberosum
Biological Phenomena
Conflict of Interest
Mammals
Manuscripts
Post Translational Protein Processing
Yeast
Proteins
Coloring Agents
Yeasts

mots-clés

    Citer ceci

    @article{06cb010f07ff4df1a2ffcf921287a744,
    title = "Proteomics analysis of the endogenous, constitutive, leaf SUMOylome",
    abstract = "SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined. Significance The authors have no conflicts of interest to declare. All authors have approved the manuscript and agree with submission to Journal of Proteomics.",
    keywords = "3D gel electrophoresis, Endogenous SUMO conjugates, Post translational modification, Solanum tuberosum, SUMOylation",
    author = "Bertrand Colignon and Edouard Delaive and Marc Dieu and Catherine Demazy and Yordan Muhovski and Cindy Wallon and Martine Raes and Sergio Mauro",
    note = "Copyright {\circledC} 2016. Published by Elsevier B.V.",
    year = "2017",
    month = "1",
    day = "6",
    doi = "10.1016/j.jprot.2016.09.012",
    language = "English",
    volume = "150",
    pages = "268--280",
    journal = "Journal of Proteomics",
    issn = "1874-3919",
    publisher = "Elsevier",

    }

    Proteomics analysis of the endogenous, constitutive, leaf SUMOylome. / Colignon, Bertrand; Delaive, Edouard; Dieu, Marc; Demazy, Catherine; Muhovski, Yordan; Wallon, Cindy; Raes, Martine; Mauro, Sergio.

    Dans: Journal of Proteomics, Vol 150, 06.01.2017, p. 268-280.

    Résultats de recherche: Contribution à un journal/une revueArticle

    TY - JOUR

    T1 - Proteomics analysis of the endogenous, constitutive, leaf SUMOylome

    AU - Colignon,Bertrand

    AU - Delaive,Edouard

    AU - Dieu,Marc

    AU - Demazy,Catherine

    AU - Muhovski,Yordan

    AU - Wallon,Cindy

    AU - Raes,Martine

    AU - Mauro,Sergio

    N1 - Copyright © 2016. Published by Elsevier B.V.

    PY - 2017/1/6

    Y1 - 2017/1/6

    N2 - SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined. Significance The authors have no conflicts of interest to declare. All authors have approved the manuscript and agree with submission to Journal of Proteomics.

    AB - SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined. Significance The authors have no conflicts of interest to declare. All authors have approved the manuscript and agree with submission to Journal of Proteomics.

    KW - 3D gel electrophoresis

    KW - Endogenous SUMO conjugates

    KW - Post translational modification

    KW - Solanum tuberosum

    KW - SUMOylation

    UR - http://www.scopus.com/inward/record.url?scp=84990943412&partnerID=8YFLogxK

    U2 - 10.1016/j.jprot.2016.09.012

    DO - 10.1016/j.jprot.2016.09.012

    M3 - Article

    VL - 150

    SP - 268

    EP - 280

    JO - Journal of Proteomics

    T2 - Journal of Proteomics

    JF - Journal of Proteomics

    SN - 1874-3919

    ER -