TY - JOUR
T1 - Comparative study of concatemer efficiency as an isotope-labelled internal standard for allergen quantification
AU - Gavage, Maxime
AU - Van Vlierberghe, Kaatje
AU - Van Poucke, C.
AU - De Loose, M.
AU - Gevaert, K.
AU - Dieu, Marc
AU - Renard, Patricia
AU - Arnould, Thierry
AU - Filee, Patrice
AU - Gillard, Nathalie
N1 - Funding Information:
The research that yielded these results was funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment through the contract RT 15/10 ALLERSENS . The authors also want to gratefully acknowledge the contributions of the University of Namur Mass Spectrometry platform (MaSUN).
Funding Information:
The research that yielded these results was funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment through the contract RT 15/10 ALLERSENS. The authors also want to gratefully acknowledge the contributions of the University of Namur Mass Spectrometry platform (MaSUN).
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Mass spectrometry-based methods coupled with stable isotope dilution have become effective and widely used methods for the detection and quantification of food allergens. Current methods target signature peptides resulting from proteolytic digestion of proteins of the allergenic ingredient. The choice of appropriate stable isotope-labelled internal standard is crucial, given the diversity of encountered food matrices which can affect sample preparation and analysis. We propose the use of concatemer, an artificial and stable isotope-labelled protein composed of several concatenated signature peptides as internal standard. With a comparative analysis of three matrices contaminated with four allergens (egg, milk, peanut, and hazelnut), the concatemer approach was found to offer advantages associated with the use of labelled proteins, ideal but unaffordable, and circumvent certain limitations of traditionally used synthetic peptides as internal standards. Although used in the proteomic field for more than a decade, concatemer strategy has not yet been applied for food analysis.
AB - Mass spectrometry-based methods coupled with stable isotope dilution have become effective and widely used methods for the detection and quantification of food allergens. Current methods target signature peptides resulting from proteolytic digestion of proteins of the allergenic ingredient. The choice of appropriate stable isotope-labelled internal standard is crucial, given the diversity of encountered food matrices which can affect sample preparation and analysis. We propose the use of concatemer, an artificial and stable isotope-labelled protein composed of several concatenated signature peptides as internal standard. With a comparative analysis of three matrices contaminated with four allergens (egg, milk, peanut, and hazelnut), the concatemer approach was found to offer advantages associated with the use of labelled proteins, ideal but unaffordable, and circumvent certain limitations of traditionally used synthetic peptides as internal standards. Although used in the proteomic field for more than a decade, concatemer strategy has not yet been applied for food analysis.
KW - Food allergen analysis
KW - Isotope dilution
KW - Isotope-labelled concatemer
KW - Isotope-labelled internal standard
KW - Mass spectrometry
UR - https://www.sciencedirect.com/science/article/pii/S0308814620312759?via%3Dihub
UR - http://www.scopus.com/inward/record.url?scp=85087513644&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2020.127413
DO - 10.1016/j.foodchem.2020.127413
M3 - Article
SN - 0308-8146
VL - 332
SP - 1
EP - 10
JO - Food Chemistry
JF - Food Chemistry
M1 - 127413
ER -