The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.
- Label-free nano LC-MS/MS
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Air pollution exposure in early life: Placental molecular changes and clinical microvascular outcomesAuthor: LUYTEN, L., 26 Mar 2021
Supervisor: Chainiaux Debacq, F. (Supervisor), Nawrot, T. (External person) (Supervisor), Colpaert, J. V. (External person) (President), ARNOULD, T. (Jury), Bautmans, I. (External person) (Jury), Gyselaers, W. (External person) (Jury), VANOIRBEEK , J. (External person) (Jury) & Vrijens, K. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences