Optimization of label-free nano LC-MS/MS analysis of the placental proteome

Leen J. Luyten; Marc Dieu; Catherine Demazy; Maude Fransolet; Tim S. Nawrot; Patricia Renard; Florence Debacq-Chainiaux

doi:10.1016/j.placenta.2020.09.013

Optimization of label-free nano LC-MS/MS analysis of the placental proteome

Leen J. Luyten, Marc Dieu, Catherine Demazy, Maude Fransolet, Tim S. Nawrot, Patricia Renard, Florence Debacq-Chainiaux

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Abstract

The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.

Original language	English
Pages (from-to)	159-162
Number of pages	4
Journal	Placenta
Volume	101
DOIs	https://doi.org/10.1016/j.placenta.2020.09.013
Publication status	Published - 10 Nov 2020

Keywords

Label-free nano LC-MS/MS
Placenta
Proteomics

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10.1016/j.placenta.2020.09.013

1-s2.0-S0143400420303052-mainFinal published version, 1.01 MBLicence: CC BY

Cite this

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title = "Optimization of label-free nano LC-MS/MS analysis of the placental proteome",

abstract = "The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.",

keywords = "Label-free nano LC-MS/MS, Placenta, Proteomics",

author = "Luyten, {Leen J.} and Marc Dieu and Catherine Demazy and Maude Fransolet and Nawrot, {Tim S.} and Patricia Renard and Florence Debacq-Chainiaux",

note = "Funding Information: Leen J. Luyten was financed by the Bijzonder Onderzoeksfonds (BOF, BOF15DOCNA01 ) partnership between the UHasselt (Diepenbeek, Belgium) and the UNamur (Namur, Belgium). Florence Debacq-Chainiaux is a Research Associate of the FNRS, Belgium. Funding Information: Leen J. Luyten was financed by the Bijzonder Onderzoeksfonds (BOF, BOF15DOCNA01) partnership between the UHasselt (Diepenbeek, Belgium) and the UNamur (Namur, Belgium). Florence Debacq-Chainiaux is a Research Associate of the FNRS, Belgium. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = nov,

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doi = "10.1016/j.placenta.2020.09.013",

language = "English",

volume = "101",

pages = "159--162",

journal = "Placenta",

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publisher = "W.B. Saunders Ltd",

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T1 - Optimization of label-free nano LC-MS/MS analysis of the placental proteome

AU - Luyten, Leen J.

AU - Dieu, Marc

AU - Demazy, Catherine

AU - Fransolet, Maude

AU - Nawrot, Tim S.

AU - Renard, Patricia

AU - Debacq-Chainiaux, Florence

N1 - Funding Information: Leen J. Luyten was financed by the Bijzonder Onderzoeksfonds (BOF, BOF15DOCNA01 ) partnership between the UHasselt (Diepenbeek, Belgium) and the UNamur (Namur, Belgium). Florence Debacq-Chainiaux is a Research Associate of the FNRS, Belgium. Funding Information: Leen J. Luyten was financed by the Bijzonder Onderzoeksfonds (BOF, BOF15DOCNA01) partnership between the UHasselt (Diepenbeek, Belgium) and the UNamur (Namur, Belgium). Florence Debacq-Chainiaux is a Research Associate of the FNRS, Belgium. Publisher Copyright: © 2020 Elsevier Ltd

PY - 2020/11/10

Y1 - 2020/11/10

N2 - The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.

AB - The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.

KW - Label-free nano LC-MS/MS

KW - Placenta

KW - Proteomics

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DO - 10.1016/j.placenta.2020.09.013

M3 - Article

AN - SCOPUS:85091573943

SN - 0143-4004

VL - 101

SP - 159

EP - 162

JO - Placenta

JF - Placenta

ER -

Optimization of label-free nano LC-MS/MS analysis of the placental proteome

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Air pollution exposure in early life: Placental molecular changes and clinical microvascular outcomes

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Optimization of label-free nano LC-MS/MS analysis of the placental proteome

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Air pollution exposure in early life: Placental molecular changes and clinical microvascular outcomes

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