Optimization of label-free nano LC-MS/MS analysis of the placental proteome

Leen J. Luyten; Marc Dieu; Catherine Demazy; Maude Fransolet; Tim S. Nawrot; Patricia Renard; Florence Debacq-Chainiaux

doi:10.1016/j.placenta.2020.09.013

Optimization of label-free nano LC-MS/MS analysis of the placental proteome

Leen J. Luyten, Marc Dieu, Catherine Demazy, Maude Fransolet, Tim S. Nawrot, Patricia Renard, Florence Debacq-Chainiaux

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Résumé

The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.

langue originale	Anglais
Pages (de - à)	159-162
Nombre de pages	4
journal	Placenta
Volume	101
Les DOIs	https://doi.org/10.1016/j.placenta.2020.09.013
Etat de la publication	Publié - 10 nov. 2020

Accès au document

10.1016/j.placenta.2020.09.013

1-s2.0-S0143400420303052-mainVersion finale publiée, 1,01 MBLicense: CC BY

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Spectrométrie de masse (MASUN)
Renard, P. (!!Manager)
Plateforme technologique Proteomique et spectrometrie de masse
Equipement/installations: Plateforme technolgique

Air pollution exposure in early life: Placental molecular changes and clinical microvascular outcomes
LUYTEN, L. (Auteur)Chainiaux Debacq, F. (Promoteur), Nawrot, T. (Promoteur), Colpaert, J. V. (Président), ARNOULD, T. (Jury), Bautmans, I. (Jury), Gyselaers, W. (Jury), VANOIRBEEK , J. (Jury) & Vrijens, K. (Jury), 26 mars 2021
Student thesis: Doc types › Docteur en Sciences

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title = "Optimization of label-free nano LC-MS/MS analysis of the placental proteome",

abstract = "The placenta can be regarded as a mirror of the events to which the fetus is exposed during development. The placental proteome has been studied with several methodologies differing in sample handling, protein extraction, and processing. We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to the conditions studied. We recommend mechanical tissue disruption, blood removal prior to protein extraction, and FASP-based or in-gel digestion.",

keywords = "Label-free nano LC-MS/MS, Placenta, Proteomics",

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note = "Funding Information: Leen J. Luyten was financed by the Bijzonder Onderzoeksfonds (BOF, BOF15DOCNA01 ) partnership between the UHasselt (Diepenbeek, Belgium) and the UNamur (Namur, Belgium). Florence Debacq-Chainiaux is a Research Associate of the FNRS, Belgium. Funding Information: Leen J. Luyten was financed by the Bijzonder Onderzoeksfonds (BOF, BOF15DOCNA01) partnership between the UHasselt (Diepenbeek, Belgium) and the UNamur (Namur, Belgium). Florence Debacq-Chainiaux is a Research Associate of the FNRS, Belgium. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

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doi = "10.1016/j.placenta.2020.09.013",

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AU - Nawrot, Tim S.

AU - Renard, Patricia

AU - Debacq-Chainiaux, Florence

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JO - Placenta

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Optimization of label-free nano LC-MS/MS analysis of the placental proteome

Résumé

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Équipement

Spectrométrie de masse (MASUN)

Thèses de l'étudiant

Air pollution exposure in early life: Placental molecular changes and clinical microvascular outcomes

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