TAPSO at low temperature

Johan Wouters, Dietmar Stalke

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

The crystal structure of TAPSO, 2-hydroxy-3-[tris-(hydroxymethyl)methylamino]propane-1-sulfonic acid, C7H17NO7S, was determined at 153 K. This widely used buffer crystallizes as a zwitterion, 2-hydroxy-3-[tris(hydroxymethyl)methylammonio]propane-l-sulfonate, and crystal cohesion is provided by a dense network of hydrogen bonds. The molecular conformation of TAPSO is compared with the structure of TRIS (or TRIZMA), another commonly used buffering agent in protein crystallization. Cell parameters reported in the literature for a series of biological buffers used in protein crystallography are also provided.

langue originaleAnglais
Pages (de - à)1684-1686
Nombre de pages3
journalActa crystallographica Section C: Crystal Structure communications
Volume52
Numéro de publication7
Etat de la publicationPublié - 15 juil. 1996

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