Abstract
The crystal structure of TAPSO, 2-hydroxy-3-[tris-(hydroxymethyl)methylamino]propane-1-sulfonic acid, C7H17NO7S, was determined at 153 K. This widely used buffer crystallizes as a zwitterion, 2-hydroxy-3-[tris(hydroxymethyl)methylammonio]propane-l-sulfonate, and crystal cohesion is provided by a dense network of hydrogen bonds. The molecular conformation of TAPSO is compared with the structure of TRIS (or TRIZMA), another commonly used buffering agent in protein crystallization. Cell parameters reported in the literature for a series of biological buffers used in protein crystallography are also provided.
| Original language | English |
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| Pages (from-to) | 1684-1686 |
| Number of pages | 3 |
| Journal | Acta crystallographica. Section C: Crystal structure communications |
| Volume | 52 |
| Issue number | 7 |
| Publication status | Published - 15 Jul 1996 |