TAPSO at low temperature

Johan Wouters, Dietmar Stalke

Research output: Contribution to journalArticlepeer-review


The crystal structure of TAPSO, 2-hydroxy-3-[tris-(hydroxymethyl)methylamino]propane-1-sulfonic acid, C7H17NO7S, was determined at 153 K. This widely used buffer crystallizes as a zwitterion, 2-hydroxy-3-[tris(hydroxymethyl)methylammonio]propane-l-sulfonate, and crystal cohesion is provided by a dense network of hydrogen bonds. The molecular conformation of TAPSO is compared with the structure of TRIS (or TRIZMA), another commonly used buffering agent in protein crystallization. Cell parameters reported in the literature for a series of biological buffers used in protein crystallography are also provided.

Original languageEnglish
Pages (from-to)1684-1686
Number of pages3
JournalActa crystallographica Section C: Crystal Structure communications
Issue number7
Publication statusPublished - 15 Jul 1996


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