Plant innate immunity offers considerable opportunities for plant protection but beside flagellin and chitin, not many molecules and their receptors have been extensively characterized and very few have successfully reached the field. COS-OGA, an elicitor that combines cationic chitosan oligomers (COS) with anionic pectin oligomers (OGA), efficiently protected tomato (Solanum lycopersicum) grown in greenhouse against powdery mildew (Leveillula taurica). Leaf proteomic analysis of plants sprayed with COS-OGA showed accumulation of Pathogenesis-Related proteins (PR), especially subtilisin-like proteases. qRT-PCR confirmed upregulation of PR-proteins and salicylic acid (SA)-related genes while expression of jasmonic acid/ethylene-associated genes was not modified. SA concentration and class III peroxidase activity were increased in leaves and appeared to be a cumulative process dependent on the number of sprayings with the elicitor. These results suggest a systemic acquired resistance (SAR) mechanism of action of the COS-OGA elicitor and highlight the importance of repeated applications to ensure efficient protection against disease.
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Patricia Renard (!!Manager)Plateforme technologique Proteomique et spectrometrie de masse
Equipement/installations: Plateforme technolgique
Thèses de l'étudiant
van Aubel, G., 13 juin 2017
Superviseur: Van Cutsem, P. (Promoteur), Messiaen, J. (Président), Legrève, A. (Personne externe) (Jury), Ongena, M. (Personne externe) (Jury), Hôfte, M. (Personne externe) (Jury) & Mauch-Mani, B. (Personne externe) (Jury)
Student thesis: Doc types › Docteur en SciencesFichier