Résumé
Introduction: Clot waveform analysis (CWA), a new methodology to assess coagulation process, can be usefully applied in various clinical settings. However, its clinical use is limited mainly because of the absence of standardization. No consensus exists regarding the wavelengths at which CWA has to be performed what is crucial for the sensitivity of the CWA. Objectives: The primary aim of this study is to determine which wavelength is the most sensitive and specific for CWA. Interindividual baseline absorbance will also be assessed as the impact of reagents from the intrinsic, extrinsic, and common coagulation pathway will be determined. Methods: Plasma samples were screened at wavelengths from 280 to 700 nm to provide absorbance spectra in clotted and nonclotted plasma. The interindividual variability of baseline absorbance was obtained by screening plasma from 50 healthy individuals at 340, 635, and 671 nm. The inner-filter effect of reagents was assessed in plasma or serum when appropriate at the same wavelengths. The reagents were those commonly used for activated partial thromboplastin time, prothrombin time, thrombin time, and dilute Russell's viper venom time. Results: Clotted plasma has higher absorbance value than nonclotted plasma (P < 0.01). The absorbance of all type of samples is higher at 340 nm than at >600 nm (P < 0.01). The interindividual variability at the different wavelengths was around 25%. However, except with the STA®-CKPrest® and STA®-NeoPTimal®, the reagents do not have a significant effect on the baseline absorbance. Conclusions: Wavelengths above 650 nm are recommended to perform CWA. Most of the commercialized reagents can be used for CWA.
| langue originale | Anglais |
|---|---|
| Pages (de - à) | 316-324 |
| Nombre de pages | 9 |
| journal | International Journal of Laboratory Hematology |
| Volume | 41 |
| Numéro de publication | 3 |
| Les DOIs | |
| état | Publié - 1 juin 2019 |
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Optimal wavelength for the clot waveform analysis : Determination of the best resolution with minimal interference of the reagents. / Evrard, Jonathan; Siriez, Romain; Morimont, Laure; Thémans, Pauline; Laloy, Julie; Bouvy, Céline; Gheldof, Damien; Mullier, François; Dogné, Jean Michel; Douxfils, Jonathan.
Dans: International Journal of Laboratory Hematology, Vol 41, Numéro 3, 01.06.2019, p. 316-324.Résultats de recherche: Contribution à un journal/une revue › Article
TY - JOUR
T1 - Optimal wavelength for the clot waveform analysis
T2 - Determination of the best resolution with minimal interference of the reagents
AU - Evrard, Jonathan
AU - Siriez, Romain
AU - Morimont, Laure
AU - Thémans, Pauline
AU - Laloy, Julie
AU - Bouvy, Céline
AU - Gheldof, Damien
AU - Mullier, François
AU - Dogné, Jean Michel
AU - Douxfils, Jonathan
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Introduction: Clot waveform analysis (CWA), a new methodology to assess coagulation process, can be usefully applied in various clinical settings. However, its clinical use is limited mainly because of the absence of standardization. No consensus exists regarding the wavelengths at which CWA has to be performed what is crucial for the sensitivity of the CWA. Objectives: The primary aim of this study is to determine which wavelength is the most sensitive and specific for CWA. Interindividual baseline absorbance will also be assessed as the impact of reagents from the intrinsic, extrinsic, and common coagulation pathway will be determined. Methods: Plasma samples were screened at wavelengths from 280 to 700 nm to provide absorbance spectra in clotted and nonclotted plasma. The interindividual variability of baseline absorbance was obtained by screening plasma from 50 healthy individuals at 340, 635, and 671 nm. The inner-filter effect of reagents was assessed in plasma or serum when appropriate at the same wavelengths. The reagents were those commonly used for activated partial thromboplastin time, prothrombin time, thrombin time, and dilute Russell's viper venom time. Results: Clotted plasma has higher absorbance value than nonclotted plasma (P < 0.01). The absorbance of all type of samples is higher at 340 nm than at >600 nm (P < 0.01). The interindividual variability at the different wavelengths was around 25%. However, except with the STA®-CKPrest® and STA®-NeoPTimal®, the reagents do not have a significant effect on the baseline absorbance. Conclusions: Wavelengths above 650 nm are recommended to perform CWA. Most of the commercialized reagents can be used for CWA.
AB - Introduction: Clot waveform analysis (CWA), a new methodology to assess coagulation process, can be usefully applied in various clinical settings. However, its clinical use is limited mainly because of the absence of standardization. No consensus exists regarding the wavelengths at which CWA has to be performed what is crucial for the sensitivity of the CWA. Objectives: The primary aim of this study is to determine which wavelength is the most sensitive and specific for CWA. Interindividual baseline absorbance will also be assessed as the impact of reagents from the intrinsic, extrinsic, and common coagulation pathway will be determined. Methods: Plasma samples were screened at wavelengths from 280 to 700 nm to provide absorbance spectra in clotted and nonclotted plasma. The interindividual variability of baseline absorbance was obtained by screening plasma from 50 healthy individuals at 340, 635, and 671 nm. The inner-filter effect of reagents was assessed in plasma or serum when appropriate at the same wavelengths. The reagents were those commonly used for activated partial thromboplastin time, prothrombin time, thrombin time, and dilute Russell's viper venom time. Results: Clotted plasma has higher absorbance value than nonclotted plasma (P < 0.01). The absorbance of all type of samples is higher at 340 nm than at >600 nm (P < 0.01). The interindividual variability at the different wavelengths was around 25%. However, except with the STA®-CKPrest® and STA®-NeoPTimal®, the reagents do not have a significant effect on the baseline absorbance. Conclusions: Wavelengths above 650 nm are recommended to perform CWA. Most of the commercialized reagents can be used for CWA.
UR - http://www.scopus.com/inward/record.url?scp=85061228679&partnerID=8YFLogxK
U2 - 10.1111/ijlh.12975
DO - 10.1111/ijlh.12975
M3 - Article
AN - SCOPUS:85061228679
VL - 41
SP - 316
EP - 324
JO - International Journal of Laboratory Hematology
JF - International Journal of Laboratory Hematology
SN - 1751-553X
IS - 3
ER -