TY - JOUR
T1 - MPV17 does not control cancer cell proliferation
AU - Canonne, Morgane
AU - Wanet, Anaïs
AU - An Nguyen, Thuy Truong
AU - Khelfi, Alexis
AU - Ayama-Canden, Sophie
AU - Van Steenbrugge, Martine
AU - Fattaccioli, Antoine
AU - Sokal, Etienne
AU - Najimi, Mustapha
AU - Arnould, Thierry
AU - Renard, Patricia
PY - 2020/3/10
Y1 - 2020/3/10
N2 - MPV17 is described as a mitochondrial inner membrane channel. Although its function remains elusive, mutations in the MPV17 gene result in hepato-cerebral mitochondrial DNA depletion syndrome in humans. In this study, we show that MPV17 silencing does not induce depletion in mitochondrial DNA content in cancer cells. We also show that MPV17 does not control cancer cell proliferation despite the fact that we initially observed a reduced proliferation rate in five MPV17-silenced cancer cell lines with two different shRNAs. How- ever, shRNA-mediated MPV17 knockdown performed in this work provided misguiding results regarding the resulting proliferation phenotype and only a rescue experiment was able to shed definitive light on the implication of MPV17 in cancer cell proliferation. Our results therefore emphasize the caution that is required when scientific conclusions are drawn from a work based on lentiviral vector-based gene silencing and clearly demonstrate the need to systematically perform a rescue experiment in order to ascertain the specific nature of the experimental results.
AB - MPV17 is described as a mitochondrial inner membrane channel. Although its function remains elusive, mutations in the MPV17 gene result in hepato-cerebral mitochondrial DNA depletion syndrome in humans. In this study, we show that MPV17 silencing does not induce depletion in mitochondrial DNA content in cancer cells. We also show that MPV17 does not control cancer cell proliferation despite the fact that we initially observed a reduced proliferation rate in five MPV17-silenced cancer cell lines with two different shRNAs. How- ever, shRNA-mediated MPV17 knockdown performed in this work provided misguiding results regarding the resulting proliferation phenotype and only a rescue experiment was able to shed definitive light on the implication of MPV17 in cancer cell proliferation. Our results therefore emphasize the caution that is required when scientific conclusions are drawn from a work based on lentiviral vector-based gene silencing and clearly demonstrate the need to systematically perform a rescue experiment in order to ascertain the specific nature of the experimental results.
KW - cancer MPV17 shRNA
UR - https:// doi.org/10.1371/journal.pone.0229834
UR - http://www.scopus.com/inward/record.url?scp=85081229179&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0229834
DO - 10.1371/journal.pone.0229834
M3 - Article
SN - 1932-6203
VL - 15
SP - 1
EP - 18
JO - PLoS ONE
JF - PLoS ONE
IS - 3
M1 - e0229834
ER -