Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer

Lucie Brisson; Piotr Bański; Martina Sboarina; Coralie Dethier; Pierre Danhier; Marie Joséphine Fontenille; Vincent F. Van Hée; Thibaut Vazeille; Morgane Tardy; Jorge Falces; Caroline Bouzin; Paolo E. Porporato; Raphaël Frédérick; Carine Michiels; Tamara Copetti; Pierre Sonveaux

doi:10.1016/j.ccell.2016.08.005

Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer

Lucie Brisson, Piotr Bański, Martina Sboarina, Coralie Dethier, Pierre Danhier, Marie Joséphine Fontenille, Vincent F. Van Hée, Thibaut Vazeille, Morgane Tardy, Jorge Falces, Caroline Bouzin, Paolo E. Porporato, Raphaël Frédérick, Carine Michiels, Tamara Copetti, Pierre Sonveaux

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Résumé

Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD⁺ to pyruvate, NADH and H⁺, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.

langue originale	Anglais
Pages (de - à)	418-431
Nombre de pages	14
journal	Cancer Cell
Volume	30
Numéro de publication	3
Les DOIs	https://doi.org/10.1016/j.ccell.2016.08.005
Etat de la publication	Publié - 2016

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10.1016/j.ccell.2016.08.005

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Brisson, L., Bański, P., Sboarina, M., Dethier, C., Danhier, P., Fontenille, M. J., Van Hée, V. F., Vazeille, T., Tardy, M., Falces, J., Bouzin, C., Porporato, P. E., Frédérick, R., Michiels, C., Copetti, T., & Sonveaux, P. (2016). Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. Cancer Cell, 30(3), 418-431. https://doi.org/10.1016/j.ccell.2016.08.005

@article{00d43cc418d34525a0819094121705c1,

title = "Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer",

abstract = "Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD+ to pyruvate, NADH and H+, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.",

author = "Lucie Brisson and Piotr Ba{\'n}ski and Martina Sboarina and Coralie Dethier and Pierre Danhier and Fontenille, {Marie Jos{\'e}phine} and {Van H{\'e}e}, {Vincent F.} and Thibaut Vazeille and Morgane Tardy and Jorge Falces and Caroline Bouzin and Porporato, {Paolo E.} and Rapha{\"e}l Fr{\'e}d{\'e}rick and Carine Michiels and Tamara Copetti and Pierre Sonveaux",

year = "2016",

doi = "10.1016/j.ccell.2016.08.005",

language = "English",

volume = "30",

pages = "418--431",

journal = "Cancer Cell",

issn = "1535-6108",

publisher = "Cell Press",

number = "3",

}

Brisson, L, Bański, P, Sboarina, M, Dethier, C, Danhier, P, Fontenille, MJ, Van Hée, VF, Vazeille, T, Tardy, M, Falces, J, Bouzin, C, Porporato, PE, Frédérick, R, Michiels, C, Copetti, T & Sonveaux, P 2016, 'Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer', Cancer Cell, VOL. 30, Numéro 3, p. 418-431. https://doi.org/10.1016/j.ccell.2016.08.005

TY - JOUR

T1 - Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer

AU - Brisson, Lucie

AU - Bański, Piotr

AU - Sboarina, Martina

AU - Dethier, Coralie

AU - Danhier, Pierre

AU - Fontenille, Marie Joséphine

AU - Van Hée, Vincent F.

AU - Vazeille, Thibaut

AU - Tardy, Morgane

AU - Falces, Jorge

AU - Bouzin, Caroline

AU - Porporato, Paolo E.

AU - Frédérick, Raphaël

AU - Michiels, Carine

AU - Copetti, Tamara

AU - Sonveaux, Pierre

PY - 2016

Y1 - 2016

N2 - Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD+ to pyruvate, NADH and H+, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.

AB - Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD+ to pyruvate, NADH and H+, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=84995602263&partnerID=8YFLogxK

U2 - 10.1016/j.ccell.2016.08.005

DO - 10.1016/j.ccell.2016.08.005

M3 - Article

AN - SCOPUS:84995602263

SN - 1535-6108

VL - 30

SP - 418

EP - 431

JO - Cancer Cell

JF - Cancer Cell

IS - 3

ER -

Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer

Résumé

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