Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer

Lucie Brisson, Piotr Bański, Martina Sboarina, Coralie Dethier, Pierre Danhier, Marie Joséphine Fontenille, Vincent F. Van Hée, Thibaut Vazeille, Morgane Tardy, Jorge Falces, Caroline Bouzin, Paolo E. Porporato, Raphaël Frédérick, Carine Michiels, Tamara Copetti, Pierre Sonveaux

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD+ to pyruvate, NADH and H+, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.

langue originaleAnglais
Pages (de - à)418-431
Nombre de pages14
journalCancer Cell
Volume30
Numéro de publication3
Les DOIs
étatPublié - 2016

Empreinte digitale

Autophagy
Lysosomes
Neoplasms
Lactic Acid
Glucose
NAD
lactate dehydrogenase 1
Starvation
Pyruvic Acid
Proteolysis
Cell Proliferation
Phenotype

Citer ceci

Brisson, L., Bański, P., Sboarina, M., Dethier, C., Danhier, P., Fontenille, M. J., ... Sonveaux, P. (2016). Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. Cancer Cell, 30(3), 418-431. https://doi.org/10.1016/j.ccell.2016.08.005
Brisson, Lucie ; Bański, Piotr ; Sboarina, Martina ; Dethier, Coralie ; Danhier, Pierre ; Fontenille, Marie Joséphine ; Van Hée, Vincent F. ; Vazeille, Thibaut ; Tardy, Morgane ; Falces, Jorge ; Bouzin, Caroline ; Porporato, Paolo E. ; Frédérick, Raphaël ; Michiels, Carine ; Copetti, Tamara ; Sonveaux, Pierre. / Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. Dans: Cancer Cell. 2016 ; Vol 30, Numéro 3. p. 418-431.
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title = "Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer",
abstract = "Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD+ to pyruvate, NADH and H+, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.",
author = "Lucie Brisson and Piotr Bański and Martina Sboarina and Coralie Dethier and Pierre Danhier and Fontenille, {Marie Jos{\'e}phine} and {Van H{\'e}e}, {Vincent F.} and Thibaut Vazeille and Morgane Tardy and Jorge Falces and Caroline Bouzin and Porporato, {Paolo E.} and Rapha{\"e}l Fr{\'e}d{\'e}rick and Carine Michiels and Tamara Copetti and Pierre Sonveaux",
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Brisson, L, Bański, P, Sboarina, M, Dethier, C, Danhier, P, Fontenille, MJ, Van Hée, VF, Vazeille, T, Tardy, M, Falces, J, Bouzin, C, Porporato, PE, Frédérick, R, Michiels, C, Copetti, T & Sonveaux, P 2016, 'Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer', Cancer Cell, VOL. 30, Numéro 3, p. 418-431. https://doi.org/10.1016/j.ccell.2016.08.005

Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. / Brisson, Lucie; Bański, Piotr; Sboarina, Martina; Dethier, Coralie; Danhier, Pierre; Fontenille, Marie Joséphine; Van Hée, Vincent F.; Vazeille, Thibaut; Tardy, Morgane; Falces, Jorge; Bouzin, Caroline; Porporato, Paolo E.; Frédérick, Raphaël; Michiels, Carine; Copetti, Tamara; Sonveaux, Pierre.

Dans: Cancer Cell, Vol 30, Numéro 3, 2016, p. 418-431.

Résultats de recherche: Contribution à un journal/une revueArticle

TY - JOUR

T1 - Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer

AU - Brisson, Lucie

AU - Bański, Piotr

AU - Sboarina, Martina

AU - Dethier, Coralie

AU - Danhier, Pierre

AU - Fontenille, Marie Joséphine

AU - Van Hée, Vincent F.

AU - Vazeille, Thibaut

AU - Tardy, Morgane

AU - Falces, Jorge

AU - Bouzin, Caroline

AU - Porporato, Paolo E.

AU - Frédérick, Raphaël

AU - Michiels, Carine

AU - Copetti, Tamara

AU - Sonveaux, Pierre

PY - 2016

Y1 - 2016

N2 - Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD+ to pyruvate, NADH and H+, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.

AB - Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD+ to pyruvate, NADH and H+, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.

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EP - 431

JO - Cancer Cell

JF - Cancer Cell

SN - 1535-6108

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Brisson L, Bański P, Sboarina M, Dethier C, Danhier P, Fontenille MJ et al. Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. Cancer Cell. 2016;30(3):418-431. https://doi.org/10.1016/j.ccell.2016.08.005