Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus

Régis Hallez; Jean-Jacques Letesson; Jean Vandenhaute; Xavier De Bolle

doi:10.1128/AEM.01873-06

Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus

Régis Hallez, Jean-Jacques Letesson, Jean Vandenhaute, Xavier De Bolle

Research output: Contribution to journal › Article › peer-review

Abstract

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

Original language	English
Pages (from-to)	1375-9
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	73
Issue number	4
DOIs	https://doi.org/10.1128/AEM.01873-06
Publication status	Published - 2007

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10.1128/AEM.01873-06

Biological Security Laboratory Level 3 (BL3)
Xavier De Bolle (Manager)
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Facility/equipment: Technological Platform

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@article{cb9cb7f8b8d44a05a25b5a79d29bc57a,

title = "Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus",

abstract = "Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.",

author = "R{\'e}gis Hallez and Jean-Jacques Letesson and Jean Vandenhaute and {De Bolle}, Xavier",

year = "2007",

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AU - Hallez, Régis

AU - Letesson, Jean-Jacques

AU - Vandenhaute, Jean

AU - De Bolle, Xavier

PY - 2007

Y1 - 2007

N2 - Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

AB - Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

U2 - 10.1128/AEM.01873-06

DO - 10.1128/AEM.01873-06

M3 - Article

C2 - 17172460

VL - 73

SP - 1375

EP - 1379

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 4

ER -

Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus

Abstract

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Biological Security Laboratory Level 3 (BL3)

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