Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus

Research output: Contribution to journalArticle

Abstract

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.
Original languageEnglish
Pages (from-to)1375-9
Number of pages5
JournalApplied and Environmental Microbiology
Volume73
Issue number4
DOIs
Publication statusPublished - 2007

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