Biological Security Laboratory Level 3 (BL3)

Facility/equipment: Technological Platform

  • Location

    61, Rue de Bruxelles




Equipments Details


A BL3 lab is a high biological security area were researchers can handle classified level3 pathogen organisms in the best security conditions. The University of Namur BL3 lab structure, equipment and procedures comply perfectly with the Walloon region Government most recent requirements (Ar. 04 juillet 2002).
The BL3 was built as a «box in the box», an independent structure built inside an existing building. This 120 m2 infrastructure, accessible through a double airlock, has 3 seclusion rooms, an animal housing facility and a shared preparation and service area. 10 researchers can work there simultaneously.
Prior to using the BL3, individuals are trained in the correct and safe use of the facility. Experimental protocols are reviewed to insure maximum safety of all planned experiments.

The Research Unit in Biology of Microorganisms (URBM) gathers 40 researchers and technicians studying microorganisms (mostly bacteria) at all levels of integration from molecular to cellular bacteriology and their relationship with eukaryotic hosts. Caulobacter crescentus, Capnocytophaga canimorsus and Brucella sp. are used as working models to study the functions, the structure and the interactions of proteins involved in cell cycle-associated processes, regulation of gene expression, metabolism and, more specifically to Brucella and Capnocytophaga canimorsus, in the modulation of the host innate immune response.

• FLUORESCENCE MICROSCOPE (air-conditioned area + digital camera)• -80°C FREEZER et 3 BIOHAZARD HOODS
• INCUBATORS (with or without CO2)

Other features:
• all the materials (partition walls, floors, furniture...) are non-porous and chemical-resistant, making cleaning and decontaminating efficient;
• the lab is maintained in constant air depression, to avoid outward leaking;
• incoming and outgoing air is constantly filtered;
• organisms are manipulated under security Class II (laminar flow) biohazard cabinets;
• the power supply is autonomous, in case of electrical failure;
• access and inter-lock control systems for the 2 airlocks;
• waste is managed and treated, notably thanks to a double entry autoclave.

Fingerprint Explore the research areas in which this equipment has been used. These labels are generated based on the related outputs. Together they form a unique fingerprint.

  • Research Output

    • 98 Article
    • 4 Chapter
    • 1 Chapter (peer-reviewed)
    • 1 Review article

    3D correlative electron microscopy reveals continuity of Brucellacontaining vacuoles with the endoplasmic reticulum

    Sedzicki, J., Tschon, T., Low, S. H., Willemart, K., Goldie, K. N., Letesson, J-J., Stahlberg, H. & Dehio, C., 1 Feb 2018, In : Journal of Cell Science. 131, 4, jcs210799.

    Research output: Contribution to journalArticle

  • Brucella central carbon metabolism: an update

    Barbier, T., Zúñiga-Ripa, A., Moussa, S., Plovier, H., Sternon, J. F., Lázaro-Antón, L., Conde-Álvarez, R., De Bolle, X., Iriarte, M., Moriyón, I. & Letesson, J. J., 4 Mar 2018, In : Critical Reviews in Microbiology. 44, 2, p. 182-211 30 p.

    Research output: Contribution to journalReview article

  • Chronic Brucella Infection Induces Selective and Persistent Interferon Gamma-Dependent Alterations of Marginal Zone Macrophages in the Spleen

    Machelart, A., Khadrawi, A., Demars, A., Willemart, K., De Trez, C., Letesson, J-J. & Muraille, E., Nov 2017, In : Infection and Immunity. 85, 11, e00115-17.

    Research output: Contribution to journalArticle