Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus

Régis Hallez; Jean-Jacques Letesson; Jean Vandenhaute; Xavier De Bolle

doi:10.1128/AEM.01873-06

Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus

Régis Hallez, Jean-Jacques Letesson, Jean Vandenhaute, Xavier De Bolle

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

langue originale	Anglais
Pages (de - à)	1375-9
Nombre de pages	5
journal	Applied and Environmental Microbiology
Volume	73
Numéro de publication	4
Les DOIs	https://doi.org/10.1128/AEM.01873-06
Etat de la publication	Publié - 2007

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10.1128/AEM.01873-06

Laboratoire de sécurité biologique au niveau 3 (BL3)
Xavier De Bolle (!!Manager)
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@article{cb9cb7f8b8d44a05a25b5a79d29bc57a,

title = "Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus",

abstract = "Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.",

author = "R{\'e}gis Hallez and Jean-Jacques Letesson and Jean Vandenhaute and {De Bolle}, Xavier",

year = "2007",

doi = "10.1128/AEM.01873-06",

language = "English",

volume = "73",

pages = "1375--9",

journal = "Applied and Environmental Microbiology",

publisher = "Wiley-Blackwell",

number = "4",

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Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus. / Hallez, Régis ; Letesson, Jean-Jacques ; Vandenhaute, Jean et al.
Dans: Applied and Environmental Microbiology, Vol 73, Numéro 4, 2007, p. 1375-9.

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

TY - JOUR

T1 - Gateway-based destination vectors for functional analyses of bacterial ORFeomes

T2 - application to the Min system in Brucella abortus

AU - Hallez, Régis

AU - Letesson, Jean-Jacques

AU - Vandenhaute, Jean

AU - De Bolle, Xavier

PY - 2007

Y1 - 2007

N2 - Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

AB - Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

U2 - 10.1128/AEM.01873-06

DO - 10.1128/AEM.01873-06

M3 - Article

C2 - 17172460

VL - 73

SP - 1375

EP - 1379

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 4

ER -

Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus

Résumé

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Laboratoire de sécurité biologique au niveau 3 (BL3)

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