Résumé
The genes encoding the restriction-modification system StyLTI of Salmonella typhimurium were inserted in vivo into the conjugative plasmid pULB21. This allowed us to transfer the StyLTI genes at a very high frequency and to monitor the fate of recipient cells after mating. Transfer of the StyLTI restriction and modification genes into a modificationless recipient was lethal and resulted in degradation of the cell's DNA. This indicates that, in contrast to any other known restriction-modification systems, StyLTI cannot be established after horizontal transfer into a naive host.
langue originale | Anglais |
---|---|
Pages (de - à) | 1328-30 |
Nombre de pages | 3 |
journal | Journal of Bacteriology |
Volume | 173 |
Numéro de publication | 3 |
Etat de la publication | Publié - 1991 |