TY - JOUR
T1 - HIF1A gene transcription is dependent on a core promoter sequence encompassing activating and inhibiting sequences located upstream from the transcription initiation site and cis elements located within the 5'UTR
AU - Minet, Emmanuel
AU - Ernest, Isabelle
AU - Michel, Gaetan
AU - Roland, Isabelle
AU - Remacle, José
AU - Raes, Martine
AU - Michiels, Carine
PY - 1999/8/2
Y1 - 1999/8/2
N2 - Hypoxia inducible factor-1 (HIF-1) is a transcription factor composed of two subunits, HIF-1α and ARNT, which is activated under hypoxia. HIF-1α mRNA is expressed constitutively in a wide variety of cell types, whereas in some others HIF1A gene expression is upregulated by hypoxia. In this report, we show that in endothelial cells (HMEC-1) the HIF-1α mRNA expression level is the same in both normoxia and hypoxia. Deletion analysis experiments of the HIF1A promoter showed that in hypoxia HIF1A gene expression is upregulated through a short sequence located next to the transcription initiation site. We also show that in hypoxia another sequence located upstream from the +1 initiation site plays an inhibitory role on HIF1A transcription in HMEC-1 but not in hepatoma cells and brings back this expression level to that observed in normoxia. Finally, we demonstrate that HIF1A gene transcription is dependent on Sp1 binding sites and that the 5'UTR sequence also contains other important cis-acting elements.
AB - Hypoxia inducible factor-1 (HIF-1) is a transcription factor composed of two subunits, HIF-1α and ARNT, which is activated under hypoxia. HIF-1α mRNA is expressed constitutively in a wide variety of cell types, whereas in some others HIF1A gene expression is upregulated by hypoxia. In this report, we show that in endothelial cells (HMEC-1) the HIF-1α mRNA expression level is the same in both normoxia and hypoxia. Deletion analysis experiments of the HIF1A promoter showed that in hypoxia HIF1A gene expression is upregulated through a short sequence located next to the transcription initiation site. We also show that in hypoxia another sequence located upstream from the +1 initiation site plays an inhibitory role on HIF1A transcription in HMEC-1 but not in hepatoma cells and brings back this expression level to that observed in normoxia. Finally, we demonstrate that HIF1A gene transcription is dependent on Sp1 binding sites and that the 5'UTR sequence also contains other important cis-acting elements.
UR - http://www.scopus.com/inward/record.url?scp=0033516997&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0995
DO - 10.1006/bbrc.1999.0995
M3 - Article
C2 - 10425220
SN - 0006-291X
VL - 261
SP - 534
EP - 540
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -