TY - JOUR
T1 - Cortical reaction as an egg quality indicator in artificial reproduction of pikeperch, Sander lucioperca
AU - Zarski, D.
AU - Krejszeff, S.
AU - Paliska, K.
AU - Targoska, K.
AU - Kupren, K.
AU - Kucharczyk, D.
AU - Fontaine, P.
AU - Kestemont, P.
N1 - MEDLINE® is the source for the MeSH terms of this document.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - The aim of this study was to investigate the process of the cortical reaction in eggs of pikeperch, Sander lucioperca (L.), as well as the application of microscopic assessment of this process in egg quality evaluation. The analysis was carried out with eggs obtained from 10 females by artificial reproduction, in which hormonal stimulation with hCG was applied. Subsequently, each sample of eggs (separately from each female fish) was analysed. The analysis included observation of the cortical reaction and the process of egg swelling, and determination of the effect of temperature (12, 14 and 16C) and the presence of spermatozoa on the cortical reaction. The results indicate that the cortical reaction in pikeperch eggs is quite violent, resulting in visible deformation of eggs between 3 and 5min after activation. No effect of temperature or the presence of spermatozoa on the cortical reaction was observed. A strong correlation was recorded for the percentage of egg deformations observed and embryo survival rate. The described method of determination of pikeperch egg quality (based on egg deformation rate between 3 and 5min after activation) may be highly useful, both in scientific research (where high-quality eggs are required) and in hatchery practice.
AB - The aim of this study was to investigate the process of the cortical reaction in eggs of pikeperch, Sander lucioperca (L.), as well as the application of microscopic assessment of this process in egg quality evaluation. The analysis was carried out with eggs obtained from 10 females by artificial reproduction, in which hormonal stimulation with hCG was applied. Subsequently, each sample of eggs (separately from each female fish) was analysed. The analysis included observation of the cortical reaction and the process of egg swelling, and determination of the effect of temperature (12, 14 and 16C) and the presence of spermatozoa on the cortical reaction. The results indicate that the cortical reaction in pikeperch eggs is quite violent, resulting in visible deformation of eggs between 3 and 5min after activation. No effect of temperature or the presence of spermatozoa on the cortical reaction was observed. A strong correlation was recorded for the percentage of egg deformations observed and embryo survival rate. The described method of determination of pikeperch egg quality (based on egg deformation rate between 3 and 5min after activation) may be highly useful, both in scientific research (where high-quality eggs are required) and in hatchery practice.
UR - http://www.scopus.com/inward/record.url?scp=84863795304&partnerID=8YFLogxK
U2 - 10.1071/RD11264
DO - 10.1071/RD11264
M3 - Article
AN - SCOPUS:84863795304
SN - 1031-3613
VL - 24
SP - 843
EP - 850
JO - Reproduction, Fertility and Development
JF - Reproduction, Fertility and Development
IS - 6
ER -