TY - JOUR
T1 - A n-3 PUFA depletion applied to rainbow trout fry (Oncorhynchus mykiss) does not modulate its subsequent lipid bioconversion capacity
AU - Mellery, Julie
AU - Brel, Jonathan
AU - Dort, Junio
AU - Geay, Florian
AU - Kestemont, Patrick
AU - Francis, David S.
AU - Larondelle, Yvan
AU - Rollin, Xavier
N1 - Publisher Copyright:
Copyright © The Authors 2017 This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/1/28
Y1 - 2017/1/28
N2 - Nutritional strategies are currently developed to produce farmed fish rich in n-3 long-chain PUFA (LC-PUFA) whilst replacing fish oil by plant-derived oils in aquafeeds. The optimisation of such strategies requires a thorough understanding of fish lipid metabolism and its nutritional modulation. The present study evaluated the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fry previously depleted in n-3 PUFA through a 60-d pre-experimental feeding period with a sunflower oil-based diet (SO) followed by a 36-d experimental period during which fish were fed either a linseed oil-based diet (LO) (this treatment being called SO/LO) or a fish oil-based diet (FO) (this treatment being called SO/FO). These treatments were compared with fish continuously fed on SO, LO or FO for 96 d. At the end of the 36-d experimental period, SO/LO and SO/FO fish recovered >80 % of the n-3 LC-PUFA reported for LO and FO fish, respectively. Fish fed on LO showed high apparent in vivo elongation and desaturation activities along the n-3 biosynthesis pathway. However, at the end of the experimental period, no impact of the fish n-3 PUFA depletion was observed on apparent in vivo elongation and desaturation activities of SO/LO fish as compared with LO fish. In contrast, the fish n-3 PUFA depletion negatively modulated the n-6 PUFA bioconversion capacity of fish in terms of reduced apparent in vivo elongation and desaturation activities. The effects were similar after 10 or 36 d of the experimental period, indicating the absence of short-term effects.
AB - Nutritional strategies are currently developed to produce farmed fish rich in n-3 long-chain PUFA (LC-PUFA) whilst replacing fish oil by plant-derived oils in aquafeeds. The optimisation of such strategies requires a thorough understanding of fish lipid metabolism and its nutritional modulation. The present study evaluated the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fry previously depleted in n-3 PUFA through a 60-d pre-experimental feeding period with a sunflower oil-based diet (SO) followed by a 36-d experimental period during which fish were fed either a linseed oil-based diet (LO) (this treatment being called SO/LO) or a fish oil-based diet (FO) (this treatment being called SO/FO). These treatments were compared with fish continuously fed on SO, LO or FO for 96 d. At the end of the 36-d experimental period, SO/LO and SO/FO fish recovered >80 % of the n-3 LC-PUFA reported for LO and FO fish, respectively. Fish fed on LO showed high apparent in vivo elongation and desaturation activities along the n-3 biosynthesis pathway. However, at the end of the experimental period, no impact of the fish n-3 PUFA depletion was observed on apparent in vivo elongation and desaturation activities of SO/LO fish as compared with LO fish. In contrast, the fish n-3 PUFA depletion negatively modulated the n-6 PUFA bioconversion capacity of fish in terms of reduced apparent in vivo elongation and desaturation activities. The effects were similar after 10 or 36 d of the experimental period, indicating the absence of short-term effects.
KW - Fatty acid metabolism
KW - Lipid bioconversion capacity
KW - Plant-derived oils
KW - Rainbow trout
KW - Whole body fatty acid balance method
KW - Fatty Acids, Omega-6/administration & dosage
KW - Lipid Metabolism
KW - Linseed Oil/metabolism
KW - Malnutrition/metabolism
KW - Fatty Acid Desaturases/metabolism
KW - Animals
KW - Sunflower Oil
KW - Diet
KW - Fish Oils/metabolism
KW - Plant Oils/metabolism
KW - Fatty Acids, Omega-3/administration & dosage
KW - Oncorhynchus mykiss/growth & development
KW - Animal Feed
KW - Aquaculture
UR - http://www.scopus.com/inward/record.url?scp=85010840084&partnerID=8YFLogxK
U2 - 10.1017/s0007114516004487
DO - 10.1017/s0007114516004487
M3 - Article
AN - SCOPUS:85010840084
SN - 0007-1145
VL - 117
SP - 187
EP - 199
JO - British Journal of Nutrition
JF - British Journal of Nutrition
IS - 2
ER -