A n-3 PUFA depletion applied to rainbow trout fry (Oncorhynchus mykiss) does not modulate its subsequent lipid bioconversion capacity

Julie Mellery, Jonathan Brel, Junio Dort, Florian Geay, Patrick Kestemont, David S. Francis, Yvan Larondelle, Xavier Rollin

Research output: Contribution to journalArticle

Abstract

Nutritional strategies are currently developed to produce farmed fish rich in n-3 long-chain PUFA (LC-PUFA) whilst replacing fish oil by plant-derived oils in aquafeeds. The optimisation of such strategies requires a thorough understanding of fish lipid metabolism and its nutritional modulation. The present study evaluated the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fry previously depleted in n-3 PUFA through a 60-d pre-experimental feeding period with a sunflower oil-based diet (SO) followed by a 36-d experimental period during which fish were fed either a linseed oil-based diet (LO) (this treatment being called SO/LO) or a fish oil-based diet (FO) (this treatment being called SO/FO). These treatments were compared with fish continuously fed on SO, LO or FO for 96 d. At the end of the 36-d experimental period, SO/LO and SO/FO fish recovered >80 % of the n-3 LC-PUFA reported for LO and FO fish, respectively. Fish fed on LO showed high apparent in vivo elongation and desaturation activities along the n-3 biosynthesis pathway. However, at the end of the experimental period, no impact of the fish n-3 PUFA depletion was observed on apparent in vivo elongation and desaturation activities of SO/LO fish as compared with LO fish. In contrast, the fish n-3 PUFA depletion negatively modulated the n-6 PUFA bioconversion capacity of fish in terms of reduced apparent in vivo elongation and desaturation activities. The effects were similar after 10 or 36 d of the experimental period, indicating the absence of short-term effects.

Original languageEnglish
Pages (from-to)187-199
Number of pages13
JournalBritish Journal of Nutrition
Volume117
Issue number2
DOIs
Publication statusPublished - 28 Jan 2017

Fingerprint

Oncorhynchus mykiss
Omega-3 Fatty Acids
Diet
Lipids
Linseed Oil
Fishes
Fish Oils
Plant Oils

Keywords

  • Fatty acid metabolism
  • Lipid bioconversion capacity
  • Plant-derived oils
  • Rainbow trout
  • Whole body fatty acid balance method
  • Fatty Acids, Omega-6/administration & dosage
  • Lipid Metabolism
  • Linseed Oil/metabolism
  • Malnutrition/metabolism
  • Fatty Acid Desaturases/metabolism
  • Animals
  • Sunflower Oil
  • Diet
  • Fish Oils/metabolism
  • Plant Oils/metabolism
  • Fatty Acids, Omega-3/administration & dosage
  • Oncorhynchus mykiss/growth & development
  • Animal Feed
  • Aquaculture

Cite this

@article{0d283fc7ad6341d599d90d8aab89b3d8,
title = "A n-3 PUFA depletion applied to rainbow trout fry (Oncorhynchus mykiss) does not modulate its subsequent lipid bioconversion capacity",
abstract = "Nutritional strategies are currently developed to produce farmed fish rich in n-3 long-chain PUFA (LC-PUFA) whilst replacing fish oil by plant-derived oils in aquafeeds. The optimisation of such strategies requires a thorough understanding of fish lipid metabolism and its nutritional modulation. The present study evaluated the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fry previously depleted in n-3 PUFA through a 60-d pre-experimental feeding period with a sunflower oil-based diet (SO) followed by a 36-d experimental period during which fish were fed either a linseed oil-based diet (LO) (this treatment being called SO/LO) or a fish oil-based diet (FO) (this treatment being called SO/FO). These treatments were compared with fish continuously fed on SO, LO or FO for 96 d. At the end of the 36-d experimental period, SO/LO and SO/FO fish recovered >80 {\%} of the n-3 LC-PUFA reported for LO and FO fish, respectively. Fish fed on LO showed high apparent in vivo elongation and desaturation activities along the n-3 biosynthesis pathway. However, at the end of the experimental period, no impact of the fish n-3 PUFA depletion was observed on apparent in vivo elongation and desaturation activities of SO/LO fish as compared with LO fish. In contrast, the fish n-3 PUFA depletion negatively modulated the n-6 PUFA bioconversion capacity of fish in terms of reduced apparent in vivo elongation and desaturation activities. The effects were similar after 10 or 36 d of the experimental period, indicating the absence of short-term effects.",
keywords = "Fatty acid metabolism, Lipid bioconversion capacity, Plant-derived oils, Rainbow trout, Whole body fatty acid balance method, Fatty Acids, Omega-6/administration & dosage, Lipid Metabolism, Linseed Oil/metabolism, Malnutrition/metabolism, Fatty Acid Desaturases/metabolism, Animals, Sunflower Oil, Diet, Fish Oils/metabolism, Plant Oils/metabolism, Fatty Acids, Omega-3/administration & dosage, Oncorhynchus mykiss/growth & development, Animal Feed, Aquaculture",
author = "Julie Mellery and Jonathan Brel and Junio Dort and Florian Geay and Patrick Kestemont and Francis, {David S.} and Yvan Larondelle and Xavier Rollin",
year = "2017",
month = "1",
day = "28",
doi = "10.1017/S0007114516004487",
language = "English",
volume = "117",
pages = "187--199",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",
number = "2",

}

A n-3 PUFA depletion applied to rainbow trout fry (Oncorhynchus mykiss) does not modulate its subsequent lipid bioconversion capacity. / Mellery, Julie; Brel, Jonathan; Dort, Junio; Geay, Florian; Kestemont, Patrick; Francis, David S.; Larondelle, Yvan; Rollin, Xavier.

In: British Journal of Nutrition, Vol. 117, No. 2, 28.01.2017, p. 187-199.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A n-3 PUFA depletion applied to rainbow trout fry (Oncorhynchus mykiss) does not modulate its subsequent lipid bioconversion capacity

AU - Mellery, Julie

AU - Brel, Jonathan

AU - Dort, Junio

AU - Geay, Florian

AU - Kestemont, Patrick

AU - Francis, David S.

AU - Larondelle, Yvan

AU - Rollin, Xavier

PY - 2017/1/28

Y1 - 2017/1/28

N2 - Nutritional strategies are currently developed to produce farmed fish rich in n-3 long-chain PUFA (LC-PUFA) whilst replacing fish oil by plant-derived oils in aquafeeds. The optimisation of such strategies requires a thorough understanding of fish lipid metabolism and its nutritional modulation. The present study evaluated the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fry previously depleted in n-3 PUFA through a 60-d pre-experimental feeding period with a sunflower oil-based diet (SO) followed by a 36-d experimental period during which fish were fed either a linseed oil-based diet (LO) (this treatment being called SO/LO) or a fish oil-based diet (FO) (this treatment being called SO/FO). These treatments were compared with fish continuously fed on SO, LO or FO for 96 d. At the end of the 36-d experimental period, SO/LO and SO/FO fish recovered >80 % of the n-3 LC-PUFA reported for LO and FO fish, respectively. Fish fed on LO showed high apparent in vivo elongation and desaturation activities along the n-3 biosynthesis pathway. However, at the end of the experimental period, no impact of the fish n-3 PUFA depletion was observed on apparent in vivo elongation and desaturation activities of SO/LO fish as compared with LO fish. In contrast, the fish n-3 PUFA depletion negatively modulated the n-6 PUFA bioconversion capacity of fish in terms of reduced apparent in vivo elongation and desaturation activities. The effects were similar after 10 or 36 d of the experimental period, indicating the absence of short-term effects.

AB - Nutritional strategies are currently developed to produce farmed fish rich in n-3 long-chain PUFA (LC-PUFA) whilst replacing fish oil by plant-derived oils in aquafeeds. The optimisation of such strategies requires a thorough understanding of fish lipid metabolism and its nutritional modulation. The present study evaluated the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fry previously depleted in n-3 PUFA through a 60-d pre-experimental feeding period with a sunflower oil-based diet (SO) followed by a 36-d experimental period during which fish were fed either a linseed oil-based diet (LO) (this treatment being called SO/LO) or a fish oil-based diet (FO) (this treatment being called SO/FO). These treatments were compared with fish continuously fed on SO, LO or FO for 96 d. At the end of the 36-d experimental period, SO/LO and SO/FO fish recovered >80 % of the n-3 LC-PUFA reported for LO and FO fish, respectively. Fish fed on LO showed high apparent in vivo elongation and desaturation activities along the n-3 biosynthesis pathway. However, at the end of the experimental period, no impact of the fish n-3 PUFA depletion was observed on apparent in vivo elongation and desaturation activities of SO/LO fish as compared with LO fish. In contrast, the fish n-3 PUFA depletion negatively modulated the n-6 PUFA bioconversion capacity of fish in terms of reduced apparent in vivo elongation and desaturation activities. The effects were similar after 10 or 36 d of the experimental period, indicating the absence of short-term effects.

KW - Fatty acid metabolism

KW - Lipid bioconversion capacity

KW - Plant-derived oils

KW - Rainbow trout

KW - Whole body fatty acid balance method

KW - Fatty Acids, Omega-6/administration & dosage

KW - Lipid Metabolism

KW - Linseed Oil/metabolism

KW - Malnutrition/metabolism

KW - Fatty Acid Desaturases/metabolism

KW - Animals

KW - Sunflower Oil

KW - Diet

KW - Fish Oils/metabolism

KW - Plant Oils/metabolism

KW - Fatty Acids, Omega-3/administration & dosage

KW - Oncorhynchus mykiss/growth & development

KW - Animal Feed

KW - Aquaculture

UR - http://www.scopus.com/inward/record.url?scp=85010840084&partnerID=8YFLogxK

U2 - 10.1017/S0007114516004487

DO - 10.1017/S0007114516004487

M3 - Article

AN - SCOPUS:85010840084

VL - 117

SP - 187

EP - 199

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 2

ER -