45th Annual Meeting of the European Society for Dermatological Research

Activité: Participation ou organisation d'un événementParticipation à une conférence, un congrès


Development and analysis of an in vitro model of epidermal infection by anthropophilic dermatophytes
Dermatophytosis, also called tinea, is a superficial lesion of the keratinized structures of the host (epidermis, nails and hairs), caused by the keratinolytic fungi named dermatophytes. Its incidence is about 10% in general population and has been increasing for the last decade, especially among some populations at risk: more than 30% of sportsmen or diabetic patients are affected. Trichophyton rubrum is an anthropophilic species responsible for 70% of human dermatophytosis, but other zoophilic species can also cause human infections, i.e. Microsporum canis and Arthroderma benhamiae. In spite of their threatening incidence, there is a lack of knowledge about the biology of dermatophytes and possibilities to counteract the mechanisms they use to develop infection. In order to study this pathology on human materials, we developed a model of infection by T. rubrum dermatophytes on reconstructed human epidermis (RHE) produced by cell culture. The infection is monitored by periodic acid-Schiff (PAS) staining method of dermatophytes and by quantitative PCR. This model allows us to study the kinetics of infection by dermatophytes and to assess the expression of pro-inflammatory cytokines by keratinocytes. The efficiency of an antifungal agent has been successfully demonstrated in this model which could thus be used to develop new curative or preventive treatments against dermatophytosis
Période9 sept. 201515 sept. 2015
Type d'événementRéunion