Single cell technologies for monitoring protein secretion heterogeneity

Fabian Stefan Franz Hartmann, Mélanie Grégoire, Francesco Renzi, Frank Delvigne

Research output: Contribution to journalReview articlepeer-review

Abstract

Cell-to-cell heterogeneity presents challenges across various fields, from biomedicine to bioproduction, where precise cellular responses are vital. While single cell technologies have significantly enhanced our understanding of population heterogeneity, the predominant focus has been on monitoring intracellular compounds. Recognizing the added complexity introduced by the secretion system, in this review, we first provide a systematic overview of the distinct steps necessary for driving protein secretion. We discuss the various sources of noise acting from the synthesized preprotein to the secretory protein released based on a Gram-positive cellular system as a model. We next explore the applicability of single cell technologies for monitoring protein secretion throughout these functional stages. We also emphasize the importance of applying these single cell technologies for monitoring protein secretion during bioproduction.

Original languageEnglish
Pages (from-to)1144-1160
Number of pages17
JournalTrends in biotechnology
Volume42
Issue number9
Early online date12 Mar 2024
DOIs
Publication statusPublished - Sept 2024

Keywords

  • biological noise
  • phenotypic heterogeneity
  • population control
  • protein secretion
  • stochasticity

Fingerprint

Dive into the research topics of 'Single cell technologies for monitoring protein secretion heterogeneity'. Together they form a unique fingerprint.

Cite this