Single cell technologies for monitoring protein secretion heterogeneity

Fabian Stefan Franz Hartmann; Mélanie Grégoire; Francesco Renzi; Frank Delvigne

doi:10.1016/j.tibtech.2024.02.011

Single cell technologies for monitoring protein secretion heterogeneity

Fabian Stefan Franz Hartmann, Mélanie Grégoire, Francesco Renzi, Frank Delvigne

University of Namur

Research output: Contribution to journal › Review article › peer-review

Abstract

Cell-to-cell heterogeneity presents challenges across various fields, from biomedicine to bioproduction, where precise cellular responses are vital. While single cell technologies have significantly enhanced our understanding of population heterogeneity, the predominant focus has been on monitoring intracellular compounds. Recognizing the added complexity introduced by the secretion system, in this review, we first provide a systematic overview of the distinct steps necessary for driving protein secretion. We discuss the various sources of noise acting from the synthesized preprotein to the secretory protein released based on a Gram-positive cellular system as a model. We next explore the applicability of single cell technologies for monitoring protein secretion throughout these functional stages. We also emphasize the importance of applying these single cell technologies for monitoring protein secretion during bioproduction.

Original language	English
Pages (from-to)	1144-1160
Number of pages	17
Journal	Trends in biotechnology
Volume	42
Issue number	9
Early online date	12 Mar 2024
DOIs	https://doi.org/10.1016/j.tibtech.2024.02.011
Publication status	Published - Sept 2024

Funding

F.H. is supported by a postdoctoral grant provided by the Service Public de Wallonie (SPW) through a Wagralim research project ‘Proactif’. M.G. is supported by a PhD grant founded by the Public Service of Wallonia (Economy, Employment ,and Research), under the FoodWal agreement n°2210182 from the Win4Excellence project of the Wallonia Recovery Plan. F.R. is a research associate (chercheur qualifié) of the Belgian Fund for Scientific Research (F.R.S.-FNRS). F.H. is supported by a postdoctoral grant provided by the Service Public de Wallonie (SPW) through a Wagralim research project ‘Proactif’. M.G. is supported by a PhD grant founded by the Public Service of Wallonia (Economy, Employment, and Research), under the FoodWal agreement n°2210182 from the Win4Excellence project of the Wallonia Recovery Plan. F.R. is a research associate (chercheur qualifié) of the Belgian Fund for Scientific Research (F.R.S.-FNRS). None declared by authors.

Funders	Funder number
Wallonia Recovery Plan
Belgian Fund for Scientific Research
Service Public de Wallonie	2210182
Service Public de Wallonie

Keywords

biological noise
phenotypic heterogeneity
population control
protein secretion
stochasticity

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10.1016/j.tibtech.2024.02.011

Cite this

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title = "Single cell technologies for monitoring protein secretion heterogeneity",

abstract = "Cell-to-cell heterogeneity presents challenges across various fields, from biomedicine to bioproduction, where precise cellular responses are vital. While single cell technologies have significantly enhanced our understanding of population heterogeneity, the predominant focus has been on monitoring intracellular compounds. Recognizing the added complexity introduced by the secretion system, in this review, we first provide a systematic overview of the distinct steps necessary for driving protein secretion. We discuss the various sources of noise acting from the synthesized preprotein to the secretory protein released based on a Gram-positive cellular system as a model. We next explore the applicability of single cell technologies for monitoring protein secretion throughout these functional stages. We also emphasize the importance of applying these single cell technologies for monitoring protein secretion during bioproduction.",

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doi = "10.1016/j.tibtech.2024.02.011",

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AU - Hartmann, Fabian Stefan Franz

AU - Grégoire, Mélanie

AU - Renzi, Francesco

AU - Delvigne, Frank

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N2 - Cell-to-cell heterogeneity presents challenges across various fields, from biomedicine to bioproduction, where precise cellular responses are vital. While single cell technologies have significantly enhanced our understanding of population heterogeneity, the predominant focus has been on monitoring intracellular compounds. Recognizing the added complexity introduced by the secretion system, in this review, we first provide a systematic overview of the distinct steps necessary for driving protein secretion. We discuss the various sources of noise acting from the synthesized preprotein to the secretory protein released based on a Gram-positive cellular system as a model. We next explore the applicability of single cell technologies for monitoring protein secretion throughout these functional stages. We also emphasize the importance of applying these single cell technologies for monitoring protein secretion during bioproduction.

AB - Cell-to-cell heterogeneity presents challenges across various fields, from biomedicine to bioproduction, where precise cellular responses are vital. While single cell technologies have significantly enhanced our understanding of population heterogeneity, the predominant focus has been on monitoring intracellular compounds. Recognizing the added complexity introduced by the secretion system, in this review, we first provide a systematic overview of the distinct steps necessary for driving protein secretion. We discuss the various sources of noise acting from the synthesized preprotein to the secretory protein released based on a Gram-positive cellular system as a model. We next explore the applicability of single cell technologies for monitoring protein secretion throughout these functional stages. We also emphasize the importance of applying these single cell technologies for monitoring protein secretion during bioproduction.

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KW - population control

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KW - stochasticity

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SP - 1144

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Single cell technologies for monitoring protein secretion heterogeneity

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Keywords

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