Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. © 2008 Elsevier Inc. All rights reserved.
|Number of pages||16|
|Journal||Free Radical Biology and Medicine|
|Publication status||Published - 15 May 2008|
- Cellular senescence
- DNA arrays
- Human fibroblasts
- Oxidative stress