Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

Florence Debacq-Chainiaux, Thierry Pascal, Emmanuelle Boilan, Coralie Bastin, Emilie Bauwens, Olivier Toussaint

Research output: Contribution to journalArticlepeer-review

Abstract

Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. © 2008 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)1817-1832
Number of pages16
JournalFree Radical Biology and Medicine
Volume44
Issue number10
DOIs
Publication statusPublished - 15 May 2008

Keywords

  • Cellular senescence
  • DNA arrays
  • Ethanol
  • Human fibroblasts
  • IGFBP-3
  • Oxidative stress
  • TGF-β1

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