Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

Florence Debacq-Chainiaux; Thierry Pascal; Emmanuelle Boilan; Coralie Bastin; Emilie Bauwens; Olivier Toussaint

doi:10.1016/j.freeradbiomed.2008.02.001

Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

Florence Debacq-Chainiaux, Thierry Pascal, Emmanuelle Boilan, Coralie Bastin, Emilie Bauwens, Olivier Toussaint

Research output: Contribution to journal › Article › peer-review

Abstract

Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. © 2008 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	1817-1832
Number of pages	16
Journal	Free Radical biology & medecine
Volume	44
Issue number	10
DOIs	https://doi.org/10.1016/j.freeradbiomed.2008.02.001
Publication status	Published - 15 May 2008

Keywords

Cellular senescence
DNA arrays
Ethanol
Human fibroblasts
IGFBP-3
Oxidative stress
TGF-β1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.freeradbiomed.2008.02.001

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title = "Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts",

abstract = "Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. {\textcopyright} 2008 Elsevier Inc. All rights reserved.",

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author = "Florence Debacq-Chainiaux and Thierry Pascal and Emmanuelle Boilan and Coralie Bastin and Emilie Bauwens and Olivier Toussaint",

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AU - Debacq-Chainiaux, Florence

AU - Pascal, Thierry

AU - Boilan, Emmanuelle

AU - Bastin, Coralie

AU - Bauwens, Emilie

AU - Toussaint, Olivier

N1 - Publication code : RES. ACAD.

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N2 - Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. © 2008 Elsevier Inc. All rights reserved.

AB - Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. © 2008 Elsevier Inc. All rights reserved.

KW - Cellular senescence

KW - DNA arrays

KW - Ethanol

KW - Human fibroblasts

KW - IGFBP-3

KW - Oxidative stress

KW - TGF-β1

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ER -

Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

Abstract

Keywords

UN SDGs

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