Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

Florence Debacq-Chainiaux; Thierry Pascal; Emmanuelle Boilan; Coralie Bastin; Emilie Bauwens; Olivier Toussaint

doi:10.1016/j.freeradbiomed.2008.02.001

Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

Florence Debacq-Chainiaux, Thierry Pascal, Emmanuelle Boilan, Coralie Bastin, Emilie Bauwens, Olivier Toussaint

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. © 2008 Elsevier Inc. All rights reserved.

langue originale	Anglais
Pages (de - à)	1817-1832
Nombre de pages	16
journal	Free Radical biology & medecine
Volume	44
Numéro de publication	10
Les DOIs	https://doi.org/10.1016/j.freeradbiomed.2008.02.001
Etat de la publication	Publié - 15 mai 2008

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10.1016/j.freeradbiomed.2008.02.001

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title = "Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts",

abstract = "Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. {\textcopyright} 2008 Elsevier Inc. All rights reserved.",

keywords = "Cellular senescence, DNA arrays, Ethanol, Human fibroblasts, IGFBP-3, Oxidative stress, TGF-β1",

author = "Florence Debacq-Chainiaux and Thierry Pascal and Emmanuelle Boilan and Coralie Bastin and Emilie Bauwens and Olivier Toussaint",

note = "Publication code : RES. ACAD.",

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Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts. / Debacq-Chainiaux, Florence; Pascal, Thierry; Boilan, Emmanuelle et al.
Dans: Free Radical biology & medecine, Vol 44, Numéro 10, 15.05.2008, p. 1817-1832.

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

TY - JOUR

T1 - Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

AU - Debacq-Chainiaux, Florence

AU - Pascal, Thierry

AU - Boilan, Emmanuelle

AU - Bastin, Coralie

AU - Bauwens, Emilie

AU - Toussaint, Olivier

N1 - Publication code : RES. ACAD.

PY - 2008/5/15

Y1 - 2008/5/15

N2 - Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. © 2008 Elsevier Inc. All rights reserved.

AB - Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol. © 2008 Elsevier Inc. All rights reserved.

KW - Cellular senescence

KW - DNA arrays

KW - Ethanol

KW - Human fibroblasts

KW - IGFBP-3

KW - Oxidative stress

KW - TGF-β1

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U2 - 10.1016/j.freeradbiomed.2008.02.001

DO - 10.1016/j.freeradbiomed.2008.02.001

M3 - Article

C2 - 18329388

SN - 1873-4596

VL - 44

SP - 1817

EP - 1832

JO - Free Radical biology & medecine

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IS - 10

ER -

Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

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