Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

Jean François Dierick; Frédéric Wenders; Florence Debacq-Chainiaux; José Remacle; Aron B. Fisher; Olivier Toussaint

doi:10.1023/A:1024154024602

Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

Jean François Dierick, Frédéric Wenders, Florence Debacq-Chainiaux, José Remacle, Aron B. Fisher, Olivier Toussaint

Research output: Contribution to journal › Article › peer-review

Abstract

In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H₂O₂-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.

Original language	English
Pages (from-to)	125-131
Number of pages	7
Journal	Biogerontology
Volume	4
Issue number	3
DOIs	https://doi.org/10.1023/A:1024154024602
Publication status	Published - 18 Jul 2003

Keywords

1-cys peroxiredoxin
Antioxidant protein 2
Cytotoxicity
Ethanol
HO
Oxidative stress
Peroxiredoxin VI
Tert-butylhydroperoxide
UVB

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title = "Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB",

abstract = "In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H2O2-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.",

keywords = "1-cys peroxiredoxin, Antioxidant protein 2, Cytotoxicity, Ethanol, HO, Oxidative stress, Peroxiredoxin VI, Tert-butylhydroperoxide, UVB",

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year = "2003",

month = jul,

day = "18",

doi = "10.1023/A:1024154024602",

language = "English",

volume = "4",

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journal = "Biogerontology",

publisher = "Springer Netherlands",

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Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB. / Dierick, Jean François; Wenders, Frédéric; Debacq-Chainiaux, Florence et al.
In: Biogerontology, Vol. 4, No. 3, 18.07.2003, p. 125-131.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

AU - Dierick, Jean François

AU - Wenders, Frédéric

AU - Debacq-Chainiaux, Florence

AU - Remacle, José

AU - Fisher, Aron B.

AU - Toussaint, Olivier

PY - 2003/7/18

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AB - In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H2O2-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.

KW - 1-cys peroxiredoxin

KW - Antioxidant protein 2

KW - Cytotoxicity

KW - Ethanol

KW - HO

KW - Oxidative stress

KW - Peroxiredoxin VI

KW - Tert-butylhydroperoxide

KW - UVB

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ER -

Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

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