Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

Jean François Dierick, Frédéric Wenders, Florence Debacq-Chainiaux, José Remacle, Aron B. Fisher, Olivier Toussaint

Research output: Contribution to journalArticlepeer-review

Abstract

In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H2O2-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.

Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalBiogerontology
Volume4
Issue number3
DOIs
Publication statusPublished - 18 Jul 2003

Keywords

  • 1-cys peroxiredoxin
  • Antioxidant protein 2
  • Cytotoxicity
  • Ethanol
  • HO
  • Oxidative stress
  • Peroxiredoxin VI
  • Tert-butylhydroperoxide
  • UVB

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