Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

Jean François Dierick; Frédéric Wenders; Florence Debacq-Chainiaux; José Remacle; Aron B. Fisher; Olivier Toussaint

doi:10.1023/A:1024154024602

Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

Jean François Dierick, Frédéric Wenders, Florence Debacq-Chainiaux, José Remacle, Aron B. Fisher, Olivier Toussaint

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H₂O₂-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.

langue originale	Anglais
Pages (de - à)	125-131
Nombre de pages	7
journal	Biogerontology
Volume	4
Numéro de publication	3
Les DOIs	https://doi.org/10.1023/A:1024154024602
Etat de la publication	Publié - 18 juil. 2003

Accès au document

10.1023/A:1024154024602

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Contient cette citation

@article{41f20339825c4e61b33cc5366d571d11,

title = "Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB",

abstract = "In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H2O2-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.",

keywords = "1-cys peroxiredoxin, Antioxidant protein 2, Cytotoxicity, Ethanol, HO, Oxidative stress, Peroxiredoxin VI, Tert-butylhydroperoxide, UVB",

author = "Dierick, {Jean Fran{\c c}ois} and Fr{\'e}d{\'e}ric Wenders and Florence Debacq-Chainiaux and Jos{\'e} Remacle and Fisher, {Aron B.} and Olivier Toussaint",

year = "2003",

month = jul,

day = "18",

doi = "10.1023/A:1024154024602",

language = "English",

volume = "4",

pages = "125--131",

journal = "Biogerontology",

publisher = "Springer Science and Business Media B.V.",

number = "3",

}

Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB. / Dierick, Jean François; Wenders, Frédéric; Debacq-Chainiaux, Florence et al.
Dans: Biogerontology, Vol 4, Numéro 3, 18.07.2003, p. 125-131.

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

TY - JOUR

T1 - Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

AU - Dierick, Jean François

AU - Wenders, Frédéric

AU - Debacq-Chainiaux, Florence

AU - Remacle, José

AU - Fisher, Aron B.

AU - Toussaint, Olivier

PY - 2003/7/18

Y1 - 2003/7/18

N2 - In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H2O2-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.

AB - In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H2O2-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.

KW - 1-cys peroxiredoxin

KW - Antioxidant protein 2

KW - Cytotoxicity

KW - Ethanol

KW - HO

KW - Oxidative stress

KW - Peroxiredoxin VI

KW - Tert-butylhydroperoxide

KW - UVB

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EP - 131

JO - Biogerontology

JF - Biogerontology

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