Key nutritional factors and interactions during larval development of pikeperch (Sander lucioperca)

Najlae El Kertaoui; Ivar Lund; Hospice Assogba; David Domínguez; Maria S. Izquierdo; Sébastien Baekelandt; Valérie Cornet; Daniel Montero; Patrick Kestemont; Syaghalirwa N M Mandiki

doi:10.1038/s41598-019-43491-1

Key nutritional factors and interactions during larval development of pikeperch (Sander lucioperca)

Najlae El Kertaoui, Ivar Lund, Hospice Assogba, David Domínguez, Maria S. Izquierdo, Sébastien Baekelandt, Valérie Cornet, Daniel Montero, Patrick Kestemont, Syaghalirwa N M Mandiki

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Abstract

The effects of 8 nutritional variables (Ca/P, Eicosapentaenoic acid (20:5n-3) + Docosahexaenoic acid (22:6n − 3) (EPA + DHA), Arachidonic acid (20:4n − 6) (ARA), Se, vitamins E, C, D and A) were investigated to identify their respective importance and interactions in pikeperch larval development. In this respect, two modalities (low and high levels) of each variable were tested through a fractional factorial experimental design allowing a reduction from 256 (2 ⁸ ) to 16 (2 ^{8 – 4} ) experimental units. Survival was significantly higher in larvae fed a high Ca/P diet while larval growth was significantly lower in larvae fed the same diet variant, associated with a higher incidence of kyphosis and pectoral anomalies in these larvae. Lordosis and scoliosis seemed to be mostly affected by dietary long chain polyunsaturated fatty acids (LC-PUFAs). A significant interaction was shown between n-3 LC-PUFA and vitamin C on jaw anomalies, while myocyte-specific enhancer factor 2C (mef2c) gene expression correlated positively with dietary vitamin C increment. Results also demonstrated an effect of the different nutrients and their interactions on the activity levels of digestive enzymatic activities. The results of the present study highlight the importance of the interactions between Ca/P, LC-PUFAs and vitamins C and E, suggesting their essential roles as key nutritional factors influencing pikeperch larval development.

Original language	English
Article number	7074
Pages (from-to)	7074
Number of pages	15
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-43491-1
Publication status	Published - 8 May 2019

Funding

The authors thank J.N. Gardeur and P. Fontaine, University of Loraine, France, for assistance with statistical analysis and Maria Jes\u00FAs Zamorano Serrano, University of Las Palmas Gran Canaria, Spain, for assistance in qPCR analysis. This study has been supported under the framework of the European Union Seventh Framework Programme project DIVERSIFY (KBBE-2013-07 single stage, GA 603121) titled \u2018Exploring the biological and socio-economic potential of new/emerging fish species for the expansion of the European aquaculture industry\u2019. A part of the research was funded by the University of Namur, Belgium through a PhD grant for N. El Kertaoui.

Funders	Funder number
University of Namur
European Commission
Seventh Framework Programme	KBBE-2013-07
Seventh Framework Programme	603121

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Key nutritional factors and interactions during larval development of pikeperch (Sander lucioperca)",

abstract = " The effects of 8 nutritional variables (Ca/P, Eicosapentaenoic acid (20:5n-3) + Docosahexaenoic acid (22:6n − 3) (EPA + DHA), Arachidonic acid (20:4n − 6) (ARA), Se, vitamins E, C, D and A) were investigated to identify their respective importance and interactions in pikeperch larval development. In this respect, two modalities (low and high levels) of each variable were tested through a fractional factorial experimental design allowing a reduction from 256 (2 8 ) to 16 (2 8 – 4 ) experimental units. Survival was significantly higher in larvae fed a high Ca/P diet while larval growth was significantly lower in larvae fed the same diet variant, associated with a higher incidence of kyphosis and pectoral anomalies in these larvae. Lordosis and scoliosis seemed to be mostly affected by dietary long chain polyunsaturated fatty acids (LC-PUFAs). A significant interaction was shown between n-3 LC-PUFA and vitamin C on jaw anomalies, while myocyte-specific enhancer factor 2C (mef2c) gene expression correlated positively with dietary vitamin C increment. Results also demonstrated an effect of the different nutrients and their interactions on the activity levels of digestive enzymatic activities. The results of the present study highlight the importance of the interactions between Ca/P, LC-PUFAs and vitamins C and E, suggesting their essential roles as key nutritional factors influencing pikeperch larval development. ",

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AU - Izquierdo, Maria S.

AU - Baekelandt, Sébastien

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Key nutritional factors and interactions during larval development of pikeperch (Sander lucioperca)

Abstract

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UN SDGs

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