Abstract
The effects of 8 nutritional variables (Ca/P, Eicosapentaenoic acid (20:5n-3) + Docosahexaenoic acid (22:6n − 3) (EPA + DHA), Arachidonic acid (20:4n − 6) (ARA), Se, vitamins E, C, D and A) were investigated to identify their respective importance and interactions in pikeperch larval development. In this respect, two modalities (low and high levels) of each variable were tested through a fractional factorial experimental design allowing a reduction from 256 (2 8 ) to 16 (2 8 – 4 ) experimental units. Survival was significantly higher in larvae fed a high Ca/P diet while larval growth was significantly lower in larvae fed the same diet variant, associated with a higher incidence of kyphosis and pectoral anomalies in these larvae. Lordosis and scoliosis seemed to be mostly affected by dietary long chain polyunsaturated fatty acids (LC-PUFAs). A significant interaction was shown between n-3 LC-PUFA and vitamin C on jaw anomalies, while myocyte-specific enhancer factor 2C (mef2c) gene expression correlated positively with dietary vitamin C increment. Results also demonstrated an effect of the different nutrients and their interactions on the activity levels of digestive enzymatic activities. The results of the present study highlight the importance of the interactions between Ca/P, LC-PUFAs and vitamins C and E, suggesting their essential roles as key nutritional factors influencing pikeperch larval development.
| Original language | English |
|---|---|
| Article number | 7074 |
| Pages (from-to) | 7074 |
| Number of pages | 15 |
| Journal | Scientific Reports |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 8 May 2019 |
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Key nutritional factors and interactions during larval development of pikeperch (Sander lucioperca). / El Kertaoui, Najlae; Lund, Ivar; Assogba, Hospice; Domínguez, David; Izquierdo, Maria S.; Baekelandt, Sébastien; Cornet, Valérie; Montero, Daniel; Kestemont, Patrick; Mandiki, Syaghalirwa N M.
In: Scientific Reports, Vol. 9, No. 1, 7074, 08.05.2019, p. 7074.Research output: Contribution to journal › Article
TY - JOUR
T1 - Key nutritional factors and interactions during larval development of pikeperch (Sander lucioperca)
AU - El Kertaoui, Najlae
AU - Lund, Ivar
AU - Assogba, Hospice
AU - Domínguez, David
AU - Izquierdo, Maria S.
AU - Baekelandt, Sébastien
AU - Cornet, Valérie
AU - Montero, Daniel
AU - Kestemont, Patrick
AU - Mandiki, Syaghalirwa N M
PY - 2019/5/8
Y1 - 2019/5/8
N2 - The effects of 8 nutritional variables (Ca/P, Eicosapentaenoic acid (20:5n-3) + Docosahexaenoic acid (22:6n − 3) (EPA + DHA), Arachidonic acid (20:4n − 6) (ARA), Se, vitamins E, C, D and A) were investigated to identify their respective importance and interactions in pikeperch larval development. In this respect, two modalities (low and high levels) of each variable were tested through a fractional factorial experimental design allowing a reduction from 256 (2 8 ) to 16 (2 8 – 4 ) experimental units. Survival was significantly higher in larvae fed a high Ca/P diet while larval growth was significantly lower in larvae fed the same diet variant, associated with a higher incidence of kyphosis and pectoral anomalies in these larvae. Lordosis and scoliosis seemed to be mostly affected by dietary long chain polyunsaturated fatty acids (LC-PUFAs). A significant interaction was shown between n-3 LC-PUFA and vitamin C on jaw anomalies, while myocyte-specific enhancer factor 2C (mef2c) gene expression correlated positively with dietary vitamin C increment. Results also demonstrated an effect of the different nutrients and their interactions on the activity levels of digestive enzymatic activities. The results of the present study highlight the importance of the interactions between Ca/P, LC-PUFAs and vitamins C and E, suggesting their essential roles as key nutritional factors influencing pikeperch larval development.
AB - The effects of 8 nutritional variables (Ca/P, Eicosapentaenoic acid (20:5n-3) + Docosahexaenoic acid (22:6n − 3) (EPA + DHA), Arachidonic acid (20:4n − 6) (ARA), Se, vitamins E, C, D and A) were investigated to identify their respective importance and interactions in pikeperch larval development. In this respect, two modalities (low and high levels) of each variable were tested through a fractional factorial experimental design allowing a reduction from 256 (2 8 ) to 16 (2 8 – 4 ) experimental units. Survival was significantly higher in larvae fed a high Ca/P diet while larval growth was significantly lower in larvae fed the same diet variant, associated with a higher incidence of kyphosis and pectoral anomalies in these larvae. Lordosis and scoliosis seemed to be mostly affected by dietary long chain polyunsaturated fatty acids (LC-PUFAs). A significant interaction was shown between n-3 LC-PUFA and vitamin C on jaw anomalies, while myocyte-specific enhancer factor 2C (mef2c) gene expression correlated positively with dietary vitamin C increment. Results also demonstrated an effect of the different nutrients and their interactions on the activity levels of digestive enzymatic activities. The results of the present study highlight the importance of the interactions between Ca/P, LC-PUFAs and vitamins C and E, suggesting their essential roles as key nutritional factors influencing pikeperch larval development.
UR - http://www.scopus.com/inward/record.url?scp=85065566177&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-43491-1
DO - 10.1038/s41598-019-43491-1
M3 - Article
C2 - 31068643
AN - SCOPUS:85065566177
VL - 9
SP - 7074
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 7074
ER -