Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Xavier Toledo-Fuentes, Céline Molinaro, Francesca Cecchet

Research output: Contribution to journalArticle


In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

Original languageEnglish
Pages (from-to)254-261
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Publication statusPublished - 1 Dec 2018


  • Light scattering
  • Lipid bilayers
  • Membranes
  • Nanoparticles (NPs)
  • Sum frequency generation (SFG) spectroscopy
  • Zeta potential

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  • Student Theses

    Study of the interactions between gold nanoparticles and models of cell membranes by second order nonlinear optical spectroscopies

    Author: Toledo-Fuentes, X., 28 Oct 2015

    Supervisor: Cecchet, F. (Supervisor), Lucas, S. (President), Caudano, Y. (Jury), Champagne, B. (Jury), Benichou, E. (External person) (Jury) & Losada-Perez, P. (External person) (Jury)

    Student thesis: Doc typesDoctor of Sciences

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