Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Xavier Toledo-Fuentes; Céline Molinaro; Francesca Cecchet

doi:10.1016/j.colsurfb.2018.08.018

Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Xavier Toledo-Fuentes, Céline Molinaro, Francesca Cecchet

Research output: Contribution to journal › Article › peer-review

Abstract

In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO ₂. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO ₂ and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

Original language	English
Pages (from-to)	254-261
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	172
DOIs	https://doi.org/10.1016/j.colsurfb.2018.08.018
Publication status	Published - 1 Dec 2018

Keywords

Light scattering
Lipid bilayers
Membranes
Nanoparticles (NPs)
Sum frequency generation (SFG) spectroscopy
Zeta potential

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10.1016/j.colsurfb.2018.08.018

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title = "Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles",

abstract = "In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs. ",

keywords = "Light scattering, Lipid bilayers, Membranes, Nanoparticles (NPs), Sum frequency generation (SFG) spectroscopy, Zeta potential",

author = "Xavier Toledo-Fuentes and C{\'e}line Molinaro and Francesca Cecchet",

note = "Funding Information: XTF and CM thank the Belgian Fund for Agricultural and Industrial Research (FRIA) and the University of Namur (UNamur), respectively, for their fellowships. FC is Research Associate of the Belgian Fund for the Scientific Research (F.R.S.-FNRS). The authors thank the COST Action MP1302 “Nanospectroscopy” and the Lasers, Optics and Spectroscopy (LOS) technological platform of UNamur. The authors acknowledge Dr. E. Perp{\`e}te (UNamur) for his help in electrophoretic and dynamic light scattering measurements. Funding Information: This work was supported by the Belgian Funds for Agricultural and Industrial Research (FRIA) and for the Scientific Research (F.R.S.-FNRS), by the University of Namur (UNamur) , and by the NATO program Science for Peace and Security under the Project 985291 “Biohazards Detection”. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

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doi = "10.1016/j.colsurfb.2018.08.018",

language = "English",

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journal = "Colloids and Surfaces B: Biointerfaces",

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AU - Toledo-Fuentes, Xavier

AU - Molinaro, Céline

AU - Cecchet, Francesca

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PY - 2018/12/1

Y1 - 2018/12/1

N2 - In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

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KW - Membranes

KW - Nanoparticles (NPs)

KW - Sum frequency generation (SFG) spectroscopy

KW - Zeta potential

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EP - 261

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Abstract

Keywords

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A novel method for the detection of biohazards

sWIMMINg: sWIMMINg. Water In Motion along Lipid Membranes: a molecular-scale study with nonlinear optical spectroscopies of the effects of the shear stress on bio-INteractions

Optics, Lasers and spectroscopy

Study of the interactions between gold nanoparticles and models of cell membranes by second order nonlinear optical spectroscopies

Cite this

Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

A novel method for the detection of biohazards

sWIMMINg: sWIMMINg. Water In Motion along Lipid Membranes: a molecular-scale study with nonlinear optical spectroscopies of the effects of the shear stress on bio-INteractions

Equipment

Optics, Lasers and spectroscopy

Student theses

Study of the interactions between gold nanoparticles and models of cell membranes by second order nonlinear optical spectroscopies

Cite this