Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Research output: Contribution to journalArticle

Abstract

In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

Original languageEnglish
Pages (from-to)254-261
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume172
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Membrane Lipids
Nanoparticles
lipids
membranes
nanoparticles
Lipid bilayers
Zeta potential
Phosphatidylcholines
Light scattering
Lipids
Fusion reactions
Gold
interactions
Spectroscopy
Membranes
Liquids
light scattering
Substrates
fusion
gold

Keywords

  • Light scattering
  • Lipid bilayers
  • Membranes
  • Nanoparticles (NPs)
  • Sum frequency generation (SFG) spectroscopy
  • Zeta potential

Cite this

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title = "Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles",
abstract = "In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.",
keywords = "Light scattering, Lipid bilayers, Membranes, Nanoparticles (NPs), Sum frequency generation (SFG) spectroscopy, Zeta potential",
author = "Xavier Toledo-Fuentes and C{\'e}line Molinaro and Francesca Cecchet",
year = "2018",
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T1 - Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

AU - Toledo-Fuentes, Xavier

AU - Molinaro, Céline

AU - Cecchet, Francesca

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N2 - In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

AB - In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

KW - Light scattering

KW - Lipid bilayers

KW - Membranes

KW - Nanoparticles (NPs)

KW - Sum frequency generation (SFG) spectroscopy

KW - Zeta potential

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