Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Xavier Toledo-Fuentes; Céline Molinaro; Francesca Cecchet

doi:10.1016/j.colsurfb.2018.08.018

Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Xavier Toledo-Fuentes, Céline Molinaro, Francesca Cecchet

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO ₂. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO ₂ and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

langue originale	Anglais
Pages (de - à)	254-261
Nombre de pages	8
journal	Colloids and Surfaces B: Biointerfaces
Volume	172
Les DOIs	https://doi.org/10.1016/j.colsurfb.2018.08.018
Etat de la publication	Publié - 1 déc. 2018

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10.1016/j.colsurfb.2018.08.018

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Link to publication in Scopus

1 Terminé
1 Pas commencé

A novel method for the detection of biohazards
Cecchet, F.
Projet: Recherche
sWIMMINg: L'eau à la surface des membranes lipidiques: étude, à l'échelle moléculaire, des effets du flux sur les interactions biologiques à l'aide des spectroscopies optiques non linéaires
Cecchet, F.
1/01/17 → 31/12/18
Projet: Recherche

Optique, Lasers et spectroscopies
Muriel Lepere (!!Manager)
Plateforme technologique Lasers, optiques et spectroscopies
Equipement/installations: Plateforme technolgique

Study of the interactions between gold nanoparticles and models of cell membranes by second order nonlinear optical spectroscopies
Auteur: Toledo-Fuentes, X., 28 oct. 2015
Superviseur: Cecchet, F. (Promoteur), Lucas, S. (Président), Caudano, Y. (Jury), Champagne, B. (Jury), Benichou, E. (Personne externe) (Jury) & Losada-Perez, P. (Personne externe) (Jury)
Student thesis: Doc types › Docteur en Sciences

Contient cette citation

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title = "Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles",

abstract = "In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs. ",

keywords = "Light scattering, Lipid bilayers, Membranes, Nanoparticles (NPs), Sum frequency generation (SFG) spectroscopy, Zeta potential",

author = "Xavier Toledo-Fuentes and C{\'e}line Molinaro and Francesca Cecchet",

note = "Funding Information: XTF and CM thank the Belgian Fund for Agricultural and Industrial Research (FRIA) and the University of Namur (UNamur), respectively, for their fellowships. FC is Research Associate of the Belgian Fund for the Scientific Research (F.R.S.-FNRS). The authors thank the COST Action MP1302 “Nanospectroscopy” and the Lasers, Optics and Spectroscopy (LOS) technological platform of UNamur. The authors acknowledge Dr. E. Perp{\`e}te (UNamur) for his help in electrophoretic and dynamic light scattering measurements. Funding Information: This work was supported by the Belgian Funds for Agricultural and Industrial Research (FRIA) and for the Scientific Research (F.R.S.-FNRS), by the University of Namur (UNamur) , and by the NATO program Science for Peace and Security under the Project 985291 “Biohazards Detection”. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

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doi = "10.1016/j.colsurfb.2018.08.018",

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AU - Toledo-Fuentes, Xavier

AU - Molinaro, Céline

AU - Cecchet, Francesca

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PY - 2018/12/1

Y1 - 2018/12/1

N2 - In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

AB - In this work, we investigated the interaction of cationic gold nanoparticles (AuNPs) with an anionic solid-supported lipid bilayer (SSLB) prepared via the spontaneous fusion of vesicles of phosphatidylserine (DPPS) and phosphatidylcholine (DPPC) on SiO 2. We combined sum frequency generation (SFG) spectroscopy at the SSLBs interfaces with electrophoretic light scattering at the vesicles/liquid interfaces, and we provided further insight into the formation of organized DPPS-DPPC films on SiO 2 and their interaction with NPs. We found that there is a critical threshold of the relative vesicles/substrate interfacial zeta potentials, beyond which the conformational organization of SSLBs failed. Moreover, we also demonstrated that the presence of anionic DPPS lipids in model mixed DPPS-DPPC membranes accelerated the interaction rate with cationic AuNPs.

KW - Light scattering

KW - Lipid bilayers

KW - Membranes

KW - Nanoparticles (NPs)

KW - Sum frequency generation (SFG) spectroscopy

KW - Zeta potential

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DO - 10.1016/j.colsurfb.2018.08.018

M3 - Article

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Interfacial charges drive the organization of supported lipid membranes and their interaction with nanoparticles

Résumé

Accès au document

Autres fichiers et liens

Empreinte digitale

Projets

A novel method for the detection of biohazards

sWIMMINg: L'eau à la surface des membranes lipidiques: étude, à l'échelle moléculaire, des effets du flux sur les interactions biologiques à l'aide des spectroscopies optiques non linéaires

Équipement

Optique, Lasers et spectroscopies

Thèses de l'étudiant

Study of the interactions between gold nanoparticles and models of cell membranes by second order nonlinear optical spectroscopies

Contient cette citation