Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis

K.Z. Boudjeltia; J. Daher; P. van Antwerpen; N. Moguilevsky; P. Delree; J. Ducobu; M. Raes; B. Badran; M. Vanhaeverbeek; D. Brohee; C. Remacle; L. Vanhamme

doi:10.1371/journal.pone.0038810

Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis

K.Z. Boudjeltia, J. Daher, P. van Antwerpen, N. Moguilevsky, P. Delree, J. Ducobu, M. Raes, B. Badran, M. Vanhaeverbeek, D. Brohee, C. Remacle, L. Vanhamme

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Abstract

Background: Blood fluidity is maintained by a delicate balance between coagulation and fibrinolysis. The endothelial cell surface is a key player in this equilibrium and cell surface disruptions can upset the balance. We investigated the role of pericellular myeloperoxidase oxidized LDLs (Mox-LDLs) in this balance. Methods and Results: We designed a technical device that enabled us to monitor fibrinolysis in real-time at the surface of an endothelial cell line (EA.hy926), and showed that Mox-LDL decreased pericellular fibrinolysis. There were no changes in fibrinolysis when EA.hy926 endothelial cells were exposed to native LDL (24 hours) at doses of 10, 50, 100 and up to 1250 μg/ml. However, treatment of EA.hy926 endothelial cells with 10 and 50 μg/ml of Mox-LDL (physiological serum concentrations) increased the lysis time by 15 and 13%, respectively (p

Original language	English
Journal	PLoS Genetics
Volume	7
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0038810
Publication status	Published - 19 Jun 2012

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title = "Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis",

abstract = "Background: Blood fluidity is maintained by a delicate balance between coagulation and fibrinolysis. The endothelial cell surface is a key player in this equilibrium and cell surface disruptions can upset the balance. We investigated the role of pericellular myeloperoxidase oxidized LDLs (Mox-LDLs) in this balance. Methods and Results: We designed a technical device that enabled us to monitor fibrinolysis in real-time at the surface of an endothelial cell line (EA.hy926), and showed that Mox-LDL decreased pericellular fibrinolysis. There were no changes in fibrinolysis when EA.hy926 endothelial cells were exposed to native LDL (24 hours) at doses of 10, 50, 100 and up to 1250 μg/ml. However, treatment of EA.hy926 endothelial cells with 10 and 50 μg/ml of Mox-LDL (physiological serum concentrations) increased the lysis time by 15 and 13%, respectively (p",

author = "K.Z. Boudjeltia and J. Daher and {van Antwerpen}, P. and N. Moguilevsky and P. Delree and J. Ducobu and M. Raes and B. Badran and M. Vanhaeverbeek and D. Brohee and C. Remacle and L. Vanhamme",

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T1 - Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis

AU - Boudjeltia, K.Z.

AU - Daher, J.

AU - van Antwerpen, P.

AU - Moguilevsky, N.

AU - Delree, P.

AU - Ducobu, J.

AU - Raes, M.

AU - Badran, B.

AU - Vanhaeverbeek, M.

AU - Brohee, D.

AU - Remacle, C.

AU - Vanhamme, L.

PY - 2012/6/19

Y1 - 2012/6/19

N2 - Background: Blood fluidity is maintained by a delicate balance between coagulation and fibrinolysis. The endothelial cell surface is a key player in this equilibrium and cell surface disruptions can upset the balance. We investigated the role of pericellular myeloperoxidase oxidized LDLs (Mox-LDLs) in this balance. Methods and Results: We designed a technical device that enabled us to monitor fibrinolysis in real-time at the surface of an endothelial cell line (EA.hy926), and showed that Mox-LDL decreased pericellular fibrinolysis. There were no changes in fibrinolysis when EA.hy926 endothelial cells were exposed to native LDL (24 hours) at doses of 10, 50, 100 and up to 1250 μg/ml. However, treatment of EA.hy926 endothelial cells with 10 and 50 μg/ml of Mox-LDL (physiological serum concentrations) increased the lysis time by 15 and 13%, respectively (p

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Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis

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