Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis

K.Z. Boudjeltia; J. Daher; P. van Antwerpen; N. Moguilevsky; P. Delree; J. Ducobu; M. Raes; B. Badran; M. Vanhaeverbeek; D. Brohee; C. Remacle; L. Vanhamme

doi:10.1371/journal.pone.0038810

Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis

K.Z. Boudjeltia, J. Daher, P. van Antwerpen, N. Moguilevsky, P. Delree, J. Ducobu, M. Raes, B. Badran, M. Vanhaeverbeek, D. Brohee, C. Remacle, L. Vanhamme

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

29 Téléchargements (Pure)

Résumé

Background: Blood fluidity is maintained by a delicate balance between coagulation and fibrinolysis. The endothelial cell surface is a key player in this equilibrium and cell surface disruptions can upset the balance. We investigated the role of pericellular myeloperoxidase oxidized LDLs (Mox-LDLs) in this balance. Methods and Results: We designed a technical device that enabled us to monitor fibrinolysis in real-time at the surface of an endothelial cell line (EA.hy926), and showed that Mox-LDL decreased pericellular fibrinolysis. There were no changes in fibrinolysis when EA.hy926 endothelial cells were exposed to native LDL (24 hours) at doses of 10, 50, 100 and up to 1250 μg/ml. However, treatment of EA.hy926 endothelial cells with 10 and 50 μg/ml of Mox-LDL (physiological serum concentrations) increased the lysis time by 15 and 13%, respectively (p

langue originale	Anglais
journal	PLoS Genetics
Volume	7
Numéro de publication	6
Les DOIs	https://doi.org/10.1371/journal.pone.0038810
Etat de la publication	Publié - 19 juin 2012

Accès au document

10.1371/journal.pone.0038810

journal.pone.0038810Version finale publiée, 756 KB

Autres fichiers et liens

Link to publication in Scopus

Contient cette citation

@article{811079149f834f9ca3229444846af5cb,

title = "Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis",

abstract = "Background: Blood fluidity is maintained by a delicate balance between coagulation and fibrinolysis. The endothelial cell surface is a key player in this equilibrium and cell surface disruptions can upset the balance. We investigated the role of pericellular myeloperoxidase oxidized LDLs (Mox-LDLs) in this balance. Methods and Results: We designed a technical device that enabled us to monitor fibrinolysis in real-time at the surface of an endothelial cell line (EA.hy926), and showed that Mox-LDL decreased pericellular fibrinolysis. There were no changes in fibrinolysis when EA.hy926 endothelial cells were exposed to native LDL (24 hours) at doses of 10, 50, 100 and up to 1250 μg/ml. However, treatment of EA.hy926 endothelial cells with 10 and 50 μg/ml of Mox-LDL (physiological serum concentrations) increased the lysis time by 15 and 13%, respectively (p",

author = "K.Z. Boudjeltia and J. Daher and {van Antwerpen}, P. and N. Moguilevsky and P. Delree and J. Ducobu and M. Raes and B. Badran and M. Vanhaeverbeek and D. Brohee and C. Remacle and L. Vanhamme",

year = "2012",

month = jun,

day = "19",

doi = "10.1371/journal.pone.0038810",

language = "English",

volume = "7",

journal = "PLoS Genetics",

issn = "1553-7390",

publisher = "Public Library of Science",

number = "6",

}

TY - JOUR

T1 - Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis

AU - Boudjeltia, K.Z.

AU - Daher, J.

AU - van Antwerpen, P.

AU - Moguilevsky, N.

AU - Delree, P.

AU - Ducobu, J.

AU - Raes, M.

AU - Badran, B.

AU - Vanhaeverbeek, M.

AU - Brohee, D.

AU - Remacle, C.

AU - Vanhamme, L.

PY - 2012/6/19

Y1 - 2012/6/19

N2 - Background: Blood fluidity is maintained by a delicate balance between coagulation and fibrinolysis. The endothelial cell surface is a key player in this equilibrium and cell surface disruptions can upset the balance. We investigated the role of pericellular myeloperoxidase oxidized LDLs (Mox-LDLs) in this balance. Methods and Results: We designed a technical device that enabled us to monitor fibrinolysis in real-time at the surface of an endothelial cell line (EA.hy926), and showed that Mox-LDL decreased pericellular fibrinolysis. There were no changes in fibrinolysis when EA.hy926 endothelial cells were exposed to native LDL (24 hours) at doses of 10, 50, 100 and up to 1250 μg/ml. However, treatment of EA.hy926 endothelial cells with 10 and 50 μg/ml of Mox-LDL (physiological serum concentrations) increased the lysis time by 15 and 13%, respectively (p

AB - Background: Blood fluidity is maintained by a delicate balance between coagulation and fibrinolysis. The endothelial cell surface is a key player in this equilibrium and cell surface disruptions can upset the balance. We investigated the role of pericellular myeloperoxidase oxidized LDLs (Mox-LDLs) in this balance. Methods and Results: We designed a technical device that enabled us to monitor fibrinolysis in real-time at the surface of an endothelial cell line (EA.hy926), and showed that Mox-LDL decreased pericellular fibrinolysis. There were no changes in fibrinolysis when EA.hy926 endothelial cells were exposed to native LDL (24 hours) at doses of 10, 50, 100 and up to 1250 μg/ml. However, treatment of EA.hy926 endothelial cells with 10 and 50 μg/ml of Mox-LDL (physiological serum concentrations) increased the lysis time by 15 and 13%, respectively (p

UR - http://www.scopus.com/inward/record.url?scp=84862546901&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0038810

DO - 10.1371/journal.pone.0038810

M3 - Article

AN - SCOPUS:84862546901

SN - 1553-7390

VL - 7

JO - PLoS Genetics

JF - PLoS Genetics

IS - 6

ER -

Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis

Résumé

Accès au document

Autres fichiers et liens

Empreinte digitale

Contient cette citation