Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum

Bertrand Colignon, Edouard Delaive, Marc Dieu, Catherine Demazy, Yordan Muhovski, Aurore Antoine, Martine Raes, Sergio Mauro

Research output: Contribution to journalArticle

Abstract

Most plant species are adapted to a relatively narrow range of ambient temperatures. Temperatures that are above the preferred range elicit a variety of responses at tissue, cellular and sub-cellular level. One of the earliest responses is post-translational modification by SUMOylation and phosphorylation; modulating heat stress (HS)-induced gene expression, metabolism adaptation and metabolite synthesis to improve plant tolerance to the heat stress. In this study we used a combination of three-dimensional gel electrophoresis fractionation, Western-blot analysis, difference in gel electrophoresis (DIGE) technology and mass spectrometry to identify those proteins that first showed changes in SUMOylation and phosphorylation in potato leaves exposed to sublethal HS. We found that these early response proteins are mostly involved in photosynthesis, or defence mechanisms, or energy metabolism. The relationship between the fractional change in SUMOylated proteoforms and in serine- phosphorylated proteoforms was not constant, but varied between proteins. The physiological significance of these differences needs further investigation.

Original languageEnglish
Pages (from-to)192-200
Number of pages9
JournalEnvironmental and Experimental Botany
Volume162
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Solanum tuberosum
heat stress
phosphorylation
gel electrophoresis
protein
electrokinesis
gel
metabolism
defense mechanism
proteins
post-translational modification
defense mechanisms
potato
energy metabolism
serine
gene expression
metabolite
fractionation
ambient temperature
photosynthesis

Keywords

  • 3D gel electrophoresis
  • DIGE
  • Heat stress
  • Proteomics
  • PTM
  • Solanum tuberosum

Cite this

@article{77d395ef28d6493e923c8e40e7ca1746,
title = "Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum",
abstract = "Most plant species are adapted to a relatively narrow range of ambient temperatures. Temperatures that are above the preferred range elicit a variety of responses at tissue, cellular and sub-cellular level. One of the earliest responses is post-translational modification by SUMOylation and phosphorylation; modulating heat stress (HS)-induced gene expression, metabolism adaptation and metabolite synthesis to improve plant tolerance to the heat stress. In this study we used a combination of three-dimensional gel electrophoresis fractionation, Western-blot analysis, difference in gel electrophoresis (DIGE) technology and mass spectrometry to identify those proteins that first showed changes in SUMOylation and phosphorylation in potato leaves exposed to sublethal HS. We found that these early response proteins are mostly involved in photosynthesis, or defence mechanisms, or energy metabolism. The relationship between the fractional change in SUMOylated proteoforms and in serine- phosphorylated proteoforms was not constant, but varied between proteins. The physiological significance of these differences needs further investigation.",
keywords = "3D gel electrophoresis, DIGE, Heat stress, Proteomics, PTM, Solanum tuberosum",
author = "Bertrand Colignon and Edouard Delaive and Marc Dieu and Catherine Demazy and Yordan Muhovski and Aurore Antoine and Martine Raes and Sergio Mauro",
year = "2019",
month = "6",
day = "1",
doi = "10.1016/j.envexpbot.2019.02.024",
language = "English",
volume = "162",
pages = "192--200",
journal = "Environmental and Experimental Botany",
issn = "0098-8472",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum

AU - Colignon, Bertrand

AU - Delaive, Edouard

AU - Dieu, Marc

AU - Demazy, Catherine

AU - Muhovski, Yordan

AU - Antoine, Aurore

AU - Raes, Martine

AU - Mauro, Sergio

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Most plant species are adapted to a relatively narrow range of ambient temperatures. Temperatures that are above the preferred range elicit a variety of responses at tissue, cellular and sub-cellular level. One of the earliest responses is post-translational modification by SUMOylation and phosphorylation; modulating heat stress (HS)-induced gene expression, metabolism adaptation and metabolite synthesis to improve plant tolerance to the heat stress. In this study we used a combination of three-dimensional gel electrophoresis fractionation, Western-blot analysis, difference in gel electrophoresis (DIGE) technology and mass spectrometry to identify those proteins that first showed changes in SUMOylation and phosphorylation in potato leaves exposed to sublethal HS. We found that these early response proteins are mostly involved in photosynthesis, or defence mechanisms, or energy metabolism. The relationship between the fractional change in SUMOylated proteoforms and in serine- phosphorylated proteoforms was not constant, but varied between proteins. The physiological significance of these differences needs further investigation.

AB - Most plant species are adapted to a relatively narrow range of ambient temperatures. Temperatures that are above the preferred range elicit a variety of responses at tissue, cellular and sub-cellular level. One of the earliest responses is post-translational modification by SUMOylation and phosphorylation; modulating heat stress (HS)-induced gene expression, metabolism adaptation and metabolite synthesis to improve plant tolerance to the heat stress. In this study we used a combination of three-dimensional gel electrophoresis fractionation, Western-blot analysis, difference in gel electrophoresis (DIGE) technology and mass spectrometry to identify those proteins that first showed changes in SUMOylation and phosphorylation in potato leaves exposed to sublethal HS. We found that these early response proteins are mostly involved in photosynthesis, or defence mechanisms, or energy metabolism. The relationship between the fractional change in SUMOylated proteoforms and in serine- phosphorylated proteoforms was not constant, but varied between proteins. The physiological significance of these differences needs further investigation.

KW - 3D gel electrophoresis

KW - DIGE

KW - Heat stress

KW - Proteomics

KW - PTM

KW - Solanum tuberosum

UR - http://www.scopus.com/inward/record.url?scp=85062225052&partnerID=8YFLogxK

U2 - 10.1016/j.envexpbot.2019.02.024

DO - 10.1016/j.envexpbot.2019.02.024

M3 - Article

VL - 162

SP - 192

EP - 200

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

ER -