Brucella melitensis invades murine erythrocytes during infection

Marie-Alice Vitry, Delphine Hanot-Mambres, Michaël Deghelt, Katrin Hack, Arnaud Machelart, Frédéric Lhomme, Jean-Marie Vanderwinden, Marjorie Vermeersch, Carl De Trez, David Pérez-Morga, Jean-Jacques Letesson, Eric Muraille

Research output: Contribution to journalArticle

65 Downloads (Pure)

Abstract

Brucella spp. are facultative intracellular Gram-negative coccobacilli responsible for brucellosis, a worldwide zoonosis. We observed that Brucella melitensis is able to persist for several weeks in the blood of intraperitoneally infected mice and that transferred blood at any time point tested is able to induce infection in naive recipient mice. Bacterial persistence in the blood is dramatically impaired by specific antibodies induced following Brucella vaccination. In contrast to Bartonella, the type IV secretion system and flagellar expression are not critically required for the persistence of Brucella in blood. ImageStream analysis of blood cells showed that following a brief extracellular phase, Brucella is associated mainly with the erythrocytes. Examination by confocal microscopy and transmission electron microscopy formally demonstrated that B. melitensis is able to invade erythrocytes in vivo. The bacteria do not seem to multiply in erythrocytes and are found free in the cytoplasm. Our results open up new areas for investigation and should serve in the development of novel strategies for the treatment or prophylaxis of brucellosis. Invasion of erythrocytes could potentially protect the bacterial cells from the host's immune response and hamper antibiotic treatment and suggests possible Brucella transmission by bloodsucking insects in nature. © 2014, American Society for Microbiology.

Original languageEnglish
Pages (from-to)3927-3938
Number of pages12
JournalInfection and Immunity
Volume82
Issue number9
DOIs
Publication statusPublished - 2014

Fingerprint

Brucella melitensis
Brucella
Erythrocytes
Infection
Brucellosis
Bartonella
Zoonoses
Microbiology
Transmission Electron Microscopy
Confocal Microscopy
Insects
Blood Cells
Cytoplasm
Vaccination
Anti-Bacterial Agents
Bacteria
Antibodies
Therapeutics

Cite this

Vitry, Marie-Alice ; Hanot-Mambres, Delphine ; Deghelt, Michaël ; Hack, Katrin ; Machelart, Arnaud ; Lhomme, Frédéric ; Vanderwinden, Jean-Marie ; Vermeersch, Marjorie ; De Trez, Carl ; Pérez-Morga, David ; Letesson, Jean-Jacques ; Muraille, Eric. / Brucella melitensis invades murine erythrocytes during infection. In: Infection and Immunity. 2014 ; Vol. 82, No. 9. pp. 3927-3938.
@article{145d998555e441d9b83604ff4bc8ec7a,
title = "Brucella melitensis invades murine erythrocytes during infection",
abstract = "Brucella spp. are facultative intracellular Gram-negative coccobacilli responsible for brucellosis, a worldwide zoonosis. We observed that Brucella melitensis is able to persist for several weeks in the blood of intraperitoneally infected mice and that transferred blood at any time point tested is able to induce infection in naive recipient mice. Bacterial persistence in the blood is dramatically impaired by specific antibodies induced following Brucella vaccination. In contrast to Bartonella, the type IV secretion system and flagellar expression are not critically required for the persistence of Brucella in blood. ImageStream analysis of blood cells showed that following a brief extracellular phase, Brucella is associated mainly with the erythrocytes. Examination by confocal microscopy and transmission electron microscopy formally demonstrated that B. melitensis is able to invade erythrocytes in vivo. The bacteria do not seem to multiply in erythrocytes and are found free in the cytoplasm. Our results open up new areas for investigation and should serve in the development of novel strategies for the treatment or prophylaxis of brucellosis. Invasion of erythrocytes could potentially protect the bacterial cells from the host's immune response and hamper antibiotic treatment and suggests possible Brucella transmission by bloodsucking insects in nature. {\circledC} 2014, American Society for Microbiology.",
author = "Marie-Alice Vitry and Delphine Hanot-Mambres and Micha{\"e}l Deghelt and Katrin Hack and Arnaud Machelart and Fr{\'e}d{\'e}ric Lhomme and Jean-Marie Vanderwinden and Marjorie Vermeersch and {De Trez}, Carl and David P{\'e}rez-Morga and Jean-Jacques Letesson and Eric Muraille",
note = "Copyright {\circledC} 2014, American Society for Microbiology. All Rights Reserved.",
year = "2014",
doi = "10.1128/IAI.01779-14",
language = "English",
volume = "82",
pages = "3927--3938",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "9",

}

Brucella melitensis invades murine erythrocytes during infection. / Vitry, Marie-Alice; Hanot-Mambres, Delphine; Deghelt, Michaël; Hack, Katrin; Machelart, Arnaud; Lhomme, Frédéric; Vanderwinden, Jean-Marie; Vermeersch, Marjorie; De Trez, Carl; Pérez-Morga, David; Letesson, Jean-Jacques; Muraille, Eric.

In: Infection and Immunity, Vol. 82, No. 9, 2014, p. 3927-3938.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Brucella melitensis invades murine erythrocytes during infection

AU - Vitry, Marie-Alice

AU - Hanot-Mambres, Delphine

AU - Deghelt, Michaël

AU - Hack, Katrin

AU - Machelart, Arnaud

AU - Lhomme, Frédéric

AU - Vanderwinden, Jean-Marie

AU - Vermeersch, Marjorie

AU - De Trez, Carl

AU - Pérez-Morga, David

AU - Letesson, Jean-Jacques

AU - Muraille, Eric

N1 - Copyright © 2014, American Society for Microbiology. All Rights Reserved.

PY - 2014

Y1 - 2014

N2 - Brucella spp. are facultative intracellular Gram-negative coccobacilli responsible for brucellosis, a worldwide zoonosis. We observed that Brucella melitensis is able to persist for several weeks in the blood of intraperitoneally infected mice and that transferred blood at any time point tested is able to induce infection in naive recipient mice. Bacterial persistence in the blood is dramatically impaired by specific antibodies induced following Brucella vaccination. In contrast to Bartonella, the type IV secretion system and flagellar expression are not critically required for the persistence of Brucella in blood. ImageStream analysis of blood cells showed that following a brief extracellular phase, Brucella is associated mainly with the erythrocytes. Examination by confocal microscopy and transmission electron microscopy formally demonstrated that B. melitensis is able to invade erythrocytes in vivo. The bacteria do not seem to multiply in erythrocytes and are found free in the cytoplasm. Our results open up new areas for investigation and should serve in the development of novel strategies for the treatment or prophylaxis of brucellosis. Invasion of erythrocytes could potentially protect the bacterial cells from the host's immune response and hamper antibiotic treatment and suggests possible Brucella transmission by bloodsucking insects in nature. © 2014, American Society for Microbiology.

AB - Brucella spp. are facultative intracellular Gram-negative coccobacilli responsible for brucellosis, a worldwide zoonosis. We observed that Brucella melitensis is able to persist for several weeks in the blood of intraperitoneally infected mice and that transferred blood at any time point tested is able to induce infection in naive recipient mice. Bacterial persistence in the blood is dramatically impaired by specific antibodies induced following Brucella vaccination. In contrast to Bartonella, the type IV secretion system and flagellar expression are not critically required for the persistence of Brucella in blood. ImageStream analysis of blood cells showed that following a brief extracellular phase, Brucella is associated mainly with the erythrocytes. Examination by confocal microscopy and transmission electron microscopy formally demonstrated that B. melitensis is able to invade erythrocytes in vivo. The bacteria do not seem to multiply in erythrocytes and are found free in the cytoplasm. Our results open up new areas for investigation and should serve in the development of novel strategies for the treatment or prophylaxis of brucellosis. Invasion of erythrocytes could potentially protect the bacterial cells from the host's immune response and hamper antibiotic treatment and suggests possible Brucella transmission by bloodsucking insects in nature. © 2014, American Society for Microbiology.

UR - http://www.scopus.com/inward/record.url?scp=84906057186&partnerID=8YFLogxK

U2 - 10.1128/IAI.01779-14

DO - 10.1128/IAI.01779-14

M3 - Article

VL - 82

SP - 3927

EP - 3938

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 9

ER -