Brucella melitensis invades murine erythrocytes during infection

Marie-Alice Vitry, Delphine Hanot-Mambres, Michaël Deghelt, Katrin Hack, Arnaud Machelart, Frédéric Lhomme, Jean-Marie Vanderwinden, Marjorie Vermeersch, Carl De Trez, David Pérez-Morga, Jean-Jacques Letesson, Eric Muraille

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Abstract

Brucella spp. are facultative intracellular Gram-negative coccobacilli responsible for brucellosis, a worldwide zoonosis. We observed that Brucella melitensis is able to persist for several weeks in the blood of intraperitoneally infected mice and that transferred blood at any time point tested is able to induce infection in naive recipient mice. Bacterial persistence in the blood is dramatically impaired by specific antibodies induced following Brucella vaccination. In contrast to Bartonella, the type IV secretion system and flagellar expression are not critically required for the persistence of Brucella in blood. ImageStream analysis of blood cells showed that following a brief extracellular phase, Brucella is associated mainly with the erythrocytes. Examination by confocal microscopy and transmission electron microscopy formally demonstrated that B. melitensis is able to invade erythrocytes in vivo. The bacteria do not seem to multiply in erythrocytes and are found free in the cytoplasm. Our results open up new areas for investigation and should serve in the development of novel strategies for the treatment or prophylaxis of brucellosis. Invasion of erythrocytes could potentially protect the bacterial cells from the host's immune response and hamper antibiotic treatment and suggests possible Brucella transmission by bloodsucking insects in nature. © 2014, American Society for Microbiology.

Original languageEnglish
Pages (from-to)3927-3938
Number of pages12
JournalInfection and Immunity
Volume82
Issue number9
DOIs
Publication statusPublished - 2014

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    Author: MacHelart, A., 2 Dec 2016

    Supervisor: Letesson, J. (Supervisor), Muraille, É. (Co-Supervisor), Cornelis, G. (President), Coutelier, J. (External person) (Jury), Gillet, L. (External person) (Jury) & Godfroid, J. (External person) (Jury)

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    Author: Vitry, M., 15 Mar 2013

    Supervisor: Letesson, J. (Supervisor), Muraille, É. (Co-Supervisor), Michiels, C. (President), Leo, O. (External person) (Jury), Goddeeris, B. (External person) (Jury) & Baldwin, C. (External person) (Jury)

    Student thesis: Doc typesDoctor of Sciences

    Cite this

    Vitry, M-A., Hanot-Mambres, D., Deghelt, M., Hack, K., Machelart, A., Lhomme, F., Vanderwinden, J-M., Vermeersch, M., De Trez, C., Pérez-Morga, D., Letesson, J-J., & Muraille, E. (2014). Brucella melitensis invades murine erythrocytes during infection. Infection and Immunity, 82(9), 3927-3938. https://doi.org/10.1128/IAI.01779-14