Projects per year
Brucella spp. are facultative intracellular Gram-negative coccobacilli responsible for brucellosis, a worldwide zoonosis. We observed that Brucella melitensis is able to persist for several weeks in the blood of intraperitoneally infected mice and that transferred blood at any time point tested is able to induce infection in naive recipient mice. Bacterial persistence in the blood is dramatically impaired by specific antibodies induced following Brucella vaccination. In contrast to Bartonella, the type IV secretion system and flagellar expression are not critically required for the persistence of Brucella in blood. ImageStream analysis of blood cells showed that following a brief extracellular phase, Brucella is associated mainly with the erythrocytes. Examination by confocal microscopy and transmission electron microscopy formally demonstrated that B. melitensis is able to invade erythrocytes in vivo. The bacteria do not seem to multiply in erythrocytes and are found free in the cytoplasm. Our results open up new areas for investigation and should serve in the development of novel strategies for the treatment or prophylaxis of brucellosis. Invasion of erythrocytes could potentially protect the bacterial cells from the host's immune response and hamper antibiotic treatment and suggests possible Brucella transmission by bloodsucking insects in nature. © 2014, American Society for Microbiology.
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PAI n°P7/28 - MICRODEV: PAI n°P7/28 - MICRODEV: Role of developmental processes in the virulence of human pathogens : from molecular mechanisms to novel therapeutic targets
1/10/12 → 30/09/17
Xavier De Bolle (Manager)Technological Platform: Biological Security Laboratory Level 3
Facility/equipment: Technological Platform
Impact de l'asthme allergique et de l'infection par Trypanosoma brucei sur le contrôle de l’infection par Brucella melitensis chez la sourisAuthor: MacHelart, A., 2 Dec 2016
Supervisor: Letesson, J. (Supervisor), Muraille, É. (Co-Supervisor), Cornelis, G. (President), Coutelier, J. (External person) (Jury), Gillet, L. (External person) (Jury) & Godfroid, J. (External person) (Jury)
Student thesis: Doc types › Doctor of SciencesFile
On the components of adaptive immune response involved in the control of a primary and secondary infection by Brucella melitensisAuthor: Vitry, M., 15 Mar 2013
Supervisor: Letesson, J. (Supervisor), Muraille, É. (Co-Supervisor), Michiels, C. (President), Leo, O. (External person) (Jury), Goddeeris, B. (External person) (Jury) & Baldwin, C. (External person) (Jury)
Student thesis: Doc types › Doctor of Sciences