Translocators YopB and YopD from Yersinia enterocolitica form a multimeric integral membrane complex in eukaryotic cell membranes

Caroline Montagner, Christian Arquint, Guy R Cornelis

    Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

    Résumé

    The type III secretion systems are contact-activated secretion systems that allow bacteria to inject effector proteins across eukaryotic cell membranes. The secretion apparatus, called injectisome or needle complex, includes a needle that terminates with a tip structure. The injectisome exports its own distal components, like the needle subunit and the needle tip. Upon contact, it exports two hydrophobic proteins called translocators (YopB and YopD in Yersinia enterocolitica) and the effectors. The translocators, assisted by the needle tip, form a pore in the target cell membrane, but the structure of this pore remains elusive. Here, we purified the membranes from infected sheep erythrocytes, and we show that they contain integrated and not simply adherent YopB and YopD. In blue native PAGE, these proteins appeared as a multimeric 500- to 700-kDa complex. This heteropolymeric YopBD complex could be copurified after solubilization in 0.5% dodecyl maltoside but not visualized in the electron microscope. We speculate that this complex may not be stable and rigid but only transient.
    langue originaleAnglais
    Pages (de - à)6923-8
    Nombre de pages6
    journalJournal of Bacteriology
    Volume193
    Numéro de publication24
    Les DOIs
    Etat de la publicationPublié - 2011

    Empreinte digitale Examiner les sujets de recherche de « Translocators YopB and YopD from Yersinia enterocolitica form a multimeric integral membrane complex in eukaryotic cell membranes ». Ensemble, ils forment une empreinte digitale unique.

    Contient cette citation