Tracer tests are widely used for characterizing hydrodynamics, from stream-scale to basin-wide scale. In karstic environments, the positioning of field fluorometers (or sampling) is mostly determined by the on-site configuration and setup difficulties. Most users are probably aware of the importance of this positioning for the relevance of data, and single-point tests are considered reliable. However, this importance is subjective to the user and the impact of positioning is not well quantified. This study aimed to quantify the spatial heterogeneity of tracer concentration through time in a karstic environment, and its impact on tracer test results and derived information on local hydrodynamics. Two approaches were considered: on-site tracing experiments in a karstic river, and Computational Fluid Dynamics (CFD) modeling of tracer dispersion through a discretized karst river channel. A comparison between on-site tracer breakthrough curves and CFD results was allowed by a thorough assessment of the river geometry. The results of on-site tracer tests showed significant heterogeneities of the breakthrough curve shape from fluorometers placed along a cross-section. CFD modeling of the tracer test through the associated discretized site geometry showed similar heterogeneity and was consistent with the positioning of on-site fluorometers, thus showing that geometry is a major contributor of the spatial heterogeneity of tracer concentration through time in karstic rivers.