Tn951 derivatives designed for high-frequency plasmid-specific transposition and deletion mutagenesis

T Michiels, G Cornelis

    Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

    Résumé

    We describe the construction of a system allowing high-frequency transposition and deletion mutagenesis with class-II transposons containing a kanamycin or a chloramphenicol-resistance marker. The system utilizes the transposition function of Tn3 and the resolution function of Tn951/Tn2501 which leads to an uncoupling of the resolution and repression functions. It consists of defective transposons inserted into conjugative, replication thermosensitive plasmids. The properties of the system are: easily selectable resistance markers, high transposition frequencies onto plasmids, low transposition frequencies onto the host chromosome, placement of the tnpA gene outside the transposons so that "second-generation" transposition does not occur, possibility to transpose the whole system onto other plasmid vectors with different selection strategies, consecutive use of two transposons for deletion mutagenesis and restriction mapping.
    langue originaleAnglais
    Pages (de - à)175-81
    Nombre de pages7
    journalGene
    Volume43
    Numéro de publication3
    Etat de la publicationPublié - 1986

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