Fluorene degradation by Sphingomonas sp. LB126 proceeds through protocatechuic acid: a genetic analysis

P Wattiau, L Bastiaens, R van Herwijnen, L Daal, J R Parsons, M E Renard, D Springael, G R Cornelis

    Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs


    Sphingomonas sp. LB126 is able to utilize fluorene as sole source of carbon and energy. In the present study, a mutagenic vector was constructed and a "plasmid rescue" strategy was set up to isolate a 16.5-kb DNA fragment containing genes required for fluorene degradation. A 14.5-kb portion of the cloned DNA was sequenced revealing thirteen open reading frames. Two encoded hypothetical proteins (FldE and FldY) similar to transcriptional regulators and one (ORF360) located on an IS-like element (ISSsp126) encoded a putative transposase. Three other putative proteins (FldB, FldU and FldV) displayed strong similarity with enzymes of the protocatechuate 4,5-degradation pathway utilized by Sphingomonaspaucimobilis SYK-6 for the degradation of lignin breakdown products. The remaining hypothetical proteins displayed only limited similarity with enzyme sequences available from databases. Suicide plasmid-directed mutagenesis and genetic complementations showed that integrity of the protocatechuate catabolic pathway was an absolute requirement for fluorene degradation to proceed. These findings were further supported by the analysis of metabolites in bacterial culture supernatants obtained from appropriate mutants. The results presented here demonstrated the suitability of the genetic tool constructed and supplied the first genetic evidence for the participation of a protocatechuate 4,5-degradation pathway in a bacterial fluorene degradation pathway.
    langue originaleAnglais
    Pages (de - à)861-72
    Nombre de pages12
    journalResearch in microbiology
    Numéro de publication10
    Etat de la publicationPublié - 2001

    Empreinte digitale

    Examiner les sujets de recherche de « Fluorene degradation by Sphingomonas sp. LB126 proceeds through protocatechuic acid: a genetic analysis ». Ensemble, ils forment une empreinte digitale unique.

    Contient cette citation