TY - GEN
T1 - Doughnut-shaped bovine serum albumin nanoparticles loaded with doxorubicin for overcoming multidrug-resistant in cancer cells
AU - Kayani, Zahra
N1 - Funding Information:
The financial supports of Research Council of University of Isfahan and Shiraz University of Medical Sciences are gratefully acknowledged.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/2
Y1 - 2018/2
N2 - Traditional spherical albumin nanoparticles remain as the dominant shape of nano-carriers described in the literature at present, due to their simple desolvation method of synthesis. However, non-spherical shapes also show great promise as cancer drug delivery vectors. In this study, we report a novel synthetic strategy based on dimethyl sulfoxide (DMSO) addition during desolvation step, to produce doughnut-shaped bovine serum albumin nanoparticles (DBSA-NPs), while maintaining narrow size distributions and homogeneity. The characteristics such as size, polydispersity and doxorubicin loading of prepared DBSA-NPs in comparison with spherical ones were determined. The biodegradation of DBSA-NPs loaded with doxorubicin (Dox-DBSA-NPs) in the presence of trypsin enzyme was spectrophotometrically monitored directly based on doxorubicin release profile. The release profile was analyzed with different kinetic models and it was best fitted with Higuchi kinetics model. The anticancer effect of Dox-DBSA-NPs against lymphoblastic leukemia (MOLT-4) and multidrug resistant uterine sarcoma (MES-SA/DX-5) cell lines were also investigated and the results were comparable with doxorubicin loaded spherical BSA nanoparticles. These results showed the potential of Dox-DBSA-NPs as a novel and high potential nano-carrier for management of non-resistance and also multidrug resistant cancer cells.
AB - Traditional spherical albumin nanoparticles remain as the dominant shape of nano-carriers described in the literature at present, due to their simple desolvation method of synthesis. However, non-spherical shapes also show great promise as cancer drug delivery vectors. In this study, we report a novel synthetic strategy based on dimethyl sulfoxide (DMSO) addition during desolvation step, to produce doughnut-shaped bovine serum albumin nanoparticles (DBSA-NPs), while maintaining narrow size distributions and homogeneity. The characteristics such as size, polydispersity and doxorubicin loading of prepared DBSA-NPs in comparison with spherical ones were determined. The biodegradation of DBSA-NPs loaded with doxorubicin (Dox-DBSA-NPs) in the presence of trypsin enzyme was spectrophotometrically monitored directly based on doxorubicin release profile. The release profile was analyzed with different kinetic models and it was best fitted with Higuchi kinetics model. The anticancer effect of Dox-DBSA-NPs against lymphoblastic leukemia (MOLT-4) and multidrug resistant uterine sarcoma (MES-SA/DX-5) cell lines were also investigated and the results were comparable with doxorubicin loaded spherical BSA nanoparticles. These results showed the potential of Dox-DBSA-NPs as a novel and high potential nano-carrier for management of non-resistance and also multidrug resistant cancer cells.
KW - Bovine serum albumin nanoparticles; Doughnut shaped nanoparticles; Doxorubicin; Multidrug resistance
KW - Multidrug resistance
KW - Doughnut shaped nanoparticles
KW - Bovine serum albumin nanoparticles
KW - Doxorubicin
UR - http://www.scopus.com/inward/record.url?scp=85033705918&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2017.10.041
DO - 10.1016/j.ijbiomac.2017.10.041
M3 - Article
SN - 0141-8130
VL - 107
SP - 1835
EP - 1843
JO - International journal of biological macromolecules
JF - International journal of biological macromolecules
ER -
