Detection of pYV+ Yersinia enterocolitica isolates by P1 slide agglutination

M P Sory; J Tollenaere; C Laszlo; T Biot; G R Cornelis; G Wauters

Detection of pYV+ Yersinia enterocolitica isolates by P1 slide agglutination

M P Sory, J Tollenaere, C Laszlo, T Biot, G R Cornelis, G Wauters

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Résumé

Rabbit polyclonal antisera were raised against the pYV-encoded outer membrane protein P1 of five Yersinia enterocolitica strains belonging to serogroups O:3, O:5,27, O:8, and O:9. Analysis of these strains with the sera showed that P1 presented at least six different antigenic factors. Two of the serum specimens were chosen to test the P1 agglutinability of 797 strains isolated from various sources. This technique appeared to be more reliable than autoagglutination and Ca2+ dependency to monitor the presence of the pYV plasmid. Hence, we propose this P1-mediated agglutination as a new and easy virulence test.

langue originale	Anglais
Pages (de - à)	2403-8
Nombre de pages	6
journal	Journal of clinical microbiology
Volume	28
Numéro de publication	11
Etat de la publication	Publié - 1990

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title = "Detection of pYV+ Yersinia enterocolitica isolates by P1 slide agglutination",

abstract = "Rabbit polyclonal antisera were raised against the pYV-encoded outer membrane protein P1 of five Yersinia enterocolitica strains belonging to serogroups O:3, O:5,27, O:8, and O:9. Analysis of these strains with the sera showed that P1 presented at least six different antigenic factors. Two of the serum specimens were chosen to test the P1 agglutinability of 797 strains isolated from various sources. This technique appeared to be more reliable than autoagglutination and Ca2+ dependency to monitor the presence of the pYV plasmid. Hence, we propose this P1-mediated agglutination as a new and easy virulence test.",

author = "Sory, {M P} and J Tollenaere and C Laszlo and T Biot and Cornelis, {G R} and G Wauters",

year = "1990",

language = "English",

volume = "28",

pages = "2403--8",

journal = "Journal of clinical microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Detection of pYV+ Yersinia enterocolitica isolates by P1 slide agglutination

AU - Sory, M P

AU - Tollenaere, J

AU - Laszlo, C

AU - Biot, T

AU - Cornelis, G R

AU - Wauters, G

PY - 1990

Y1 - 1990

N2 - Rabbit polyclonal antisera were raised against the pYV-encoded outer membrane protein P1 of five Yersinia enterocolitica strains belonging to serogroups O:3, O:5,27, O:8, and O:9. Analysis of these strains with the sera showed that P1 presented at least six different antigenic factors. Two of the serum specimens were chosen to test the P1 agglutinability of 797 strains isolated from various sources. This technique appeared to be more reliable than autoagglutination and Ca2+ dependency to monitor the presence of the pYV plasmid. Hence, we propose this P1-mediated agglutination as a new and easy virulence test.

AB - Rabbit polyclonal antisera were raised against the pYV-encoded outer membrane protein P1 of five Yersinia enterocolitica strains belonging to serogroups O:3, O:5,27, O:8, and O:9. Analysis of these strains with the sera showed that P1 presented at least six different antigenic factors. Two of the serum specimens were chosen to test the P1 agglutinability of 797 strains isolated from various sources. This technique appeared to be more reliable than autoagglutination and Ca2+ dependency to monitor the presence of the pYV plasmid. Hence, we propose this P1-mediated agglutination as a new and easy virulence test.

M3 - Article

C2 - 2254416

SN - 0095-1137

VL - 28

SP - 2403

EP - 2408

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 11

ER -

Detection of pYV+ Yersinia enterocolitica isolates by P1 slide agglutination

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