Assessment of a computed tomography guided injection technique of the lumbo-sacral disc in sheep

Fabienne Neveu, Jean Michel Vandeweerd, Nathalie Kirschvink, Kadhija Nozry, Pascal Gustin, Alex Dugdale, Benoît Bihin, Jean François Nisolle

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Objectives: Recent data indicate that degeneration of intervertebral discs occurs naturally in sheep, with a higher prevalence at the level of the lumbo-sacral disc. The objective of this ex vivo study was to evaluate a computed tomography (CT) guided method of injection into the ovine lumbo-sacral disc. Methods: Six euthanatized sheep were used for identification of the approach plane, the optimal direction of the needle and the mean distance from skin to disc. Dissection after injection of coloured ink was used to determine the anatomical structures that were penetrated. In seven other animals, all spines were assessed beforehand by CT and magnetic resonance imaging to determine whether disc pathology was present. The final position of the needle was assessed by CT to determine the accuracy of the technique. Contrast agent was injected to identify any problems associated with administration of liquid into the disc. Results: The CT guided injection technique was easy to perform and enabled adequate positioning of the needle into all (n = 7) lumbo-sacral discs. Distance between the skin and the disc ranged between 12 and 17 cm. No organ, vascular or nervous structure was penetrated and the needle path remained intramuscular without penetration of the peritoneal cavity. Contrast medium leaked out through three degenerate discs. Clinical significance: The current study described a consistently safe and accurate CT guided injection technique to the lumbosacral disc for future in vivo experimental studies that will use sheep as animal model for human intervertebral disc disease disease.

langueAnglais
Pages136-141
Nombre de pages6
journalVeterinary and Comparative Orthopaedics and Traumatology
Volume29
Numéro2
Les DOIs
étatPublié - 2016

Empreinte digitale

Sheep
Tomography
Injections
computed tomography
injection
sheep
methodology
Needles
Contrast Media
Skin
intervertebral disks
skin (animal)
Intervertebral Disc Degeneration
Ink
Peritoneal Cavity
Blood Vessels
Dissection
Spine
Animal Models
Magnetic Resonance Imaging

mots-clés

    Citer ceci

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    title = "Assessment of a computed tomography guided injection technique of the lumbo-sacral disc in sheep",
    abstract = "Objectives: Recent data indicate that degeneration of intervertebral discs occurs naturally in sheep, with a higher prevalence at the level of the lumbo-sacral disc. The objective of this ex vivo study was to evaluate a computed tomography (CT) guided method of injection into the ovine lumbo-sacral disc. Methods: Six euthanatized sheep were used for identification of the approach plane, the optimal direction of the needle and the mean distance from skin to disc. Dissection after injection of coloured ink was used to determine the anatomical structures that were penetrated. In seven other animals, all spines were assessed beforehand by CT and magnetic resonance imaging to determine whether disc pathology was present. The final position of the needle was assessed by CT to determine the accuracy of the technique. Contrast agent was injected to identify any problems associated with administration of liquid into the disc. Results: The CT guided injection technique was easy to perform and enabled adequate positioning of the needle into all (n = 7) lumbo-sacral discs. Distance between the skin and the disc ranged between 12 and 17 cm. No organ, vascular or nervous structure was penetrated and the needle path remained intramuscular without penetration of the peritoneal cavity. Contrast medium leaked out through three degenerate discs. Clinical significance: The current study described a consistently safe and accurate CT guided injection technique to the lumbosacral disc for future in vivo experimental studies that will use sheep as animal model for human intervertebral disc disease disease.",
    keywords = "Computed tomography, CT, Injection, Intervertebral disc, Lumbosacral, Sheep",
    author = "Fabienne Neveu and Vandeweerd, {Jean Michel} and Nathalie Kirschvink and Kadhija Nozry and Pascal Gustin and Alex Dugdale and Beno\{^i}t Bihin and Nisolle, {Jean Fran\{cc}ois}",
    year = "2016",
    doi = "10.3415/VCOT-15-06-0100",
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    volume = "29",
    pages = "136--141",
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    issn = "0932-0814",
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    Assessment of a computed tomography guided injection technique of the lumbo-sacral disc in sheep. / Neveu, Fabienne; Vandeweerd, Jean Michel; Kirschvink, Nathalie; Nozry, Kadhija; Gustin, Pascal; Dugdale, Alex; Bihin, Benoît; Nisolle, Jean François.

    Dans: Veterinary and Comparative Orthopaedics and Traumatology, Vol 29, Numéro 2, 2016, p. 136-141.

    Résultats de recherche: Contribution à un journal/une revueArticle

    TY - JOUR

    T1 - Assessment of a computed tomography guided injection technique of the lumbo-sacral disc in sheep

    AU - Neveu,Fabienne

    AU - Vandeweerd,Jean Michel

    AU - Kirschvink,Nathalie

    AU - Nozry,Kadhija

    AU - Gustin,Pascal

    AU - Dugdale,Alex

    AU - Bihin,Benoît

    AU - Nisolle,Jean François

    PY - 2016

    Y1 - 2016

    N2 - Objectives: Recent data indicate that degeneration of intervertebral discs occurs naturally in sheep, with a higher prevalence at the level of the lumbo-sacral disc. The objective of this ex vivo study was to evaluate a computed tomography (CT) guided method of injection into the ovine lumbo-sacral disc. Methods: Six euthanatized sheep were used for identification of the approach plane, the optimal direction of the needle and the mean distance from skin to disc. Dissection after injection of coloured ink was used to determine the anatomical structures that were penetrated. In seven other animals, all spines were assessed beforehand by CT and magnetic resonance imaging to determine whether disc pathology was present. The final position of the needle was assessed by CT to determine the accuracy of the technique. Contrast agent was injected to identify any problems associated with administration of liquid into the disc. Results: The CT guided injection technique was easy to perform and enabled adequate positioning of the needle into all (n = 7) lumbo-sacral discs. Distance between the skin and the disc ranged between 12 and 17 cm. No organ, vascular or nervous structure was penetrated and the needle path remained intramuscular without penetration of the peritoneal cavity. Contrast medium leaked out through three degenerate discs. Clinical significance: The current study described a consistently safe and accurate CT guided injection technique to the lumbosacral disc for future in vivo experimental studies that will use sheep as animal model for human intervertebral disc disease disease.

    AB - Objectives: Recent data indicate that degeneration of intervertebral discs occurs naturally in sheep, with a higher prevalence at the level of the lumbo-sacral disc. The objective of this ex vivo study was to evaluate a computed tomography (CT) guided method of injection into the ovine lumbo-sacral disc. Methods: Six euthanatized sheep were used for identification of the approach plane, the optimal direction of the needle and the mean distance from skin to disc. Dissection after injection of coloured ink was used to determine the anatomical structures that were penetrated. In seven other animals, all spines were assessed beforehand by CT and magnetic resonance imaging to determine whether disc pathology was present. The final position of the needle was assessed by CT to determine the accuracy of the technique. Contrast agent was injected to identify any problems associated with administration of liquid into the disc. Results: The CT guided injection technique was easy to perform and enabled adequate positioning of the needle into all (n = 7) lumbo-sacral discs. Distance between the skin and the disc ranged between 12 and 17 cm. No organ, vascular or nervous structure was penetrated and the needle path remained intramuscular without penetration of the peritoneal cavity. Contrast medium leaked out through three degenerate discs. Clinical significance: The current study described a consistently safe and accurate CT guided injection technique to the lumbosacral disc for future in vivo experimental studies that will use sheep as animal model for human intervertebral disc disease disease.

    KW - Computed tomography

    KW - CT

    KW - Injection

    KW - Intervertebral disc

    KW - Lumbosacral

    KW - Sheep

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    M3 - Article

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    EP - 141

    JO - Veterinary and Comparative Orthopaedics and Traumatology

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