Biodiversity used within the context of plant enhancement presupposes an analysis of the locally existing genotypes adapted to particular environments. Within the scope of a pigeon pea enhancement project, we carried out the exploration and collection of pigeon pea landraces from Lambayeque, department located at the north of Peru. Then, we proceeded to the agro-morphological characterization of the maternal families (derived from these landraces) based on descriptors. At the same time, we undertook the molecular characterization of the collection, as well as in some Indian and African accessions, by using microsatellites markers developed by us. We also used microsatellites provided by the ICRISAT. The molecular characterization is an important stage, which requires low-cost precision instruments, efficient and easy to use. One of them are the microsatellites, codominant markers. Thanks to these seven microsatellite markers, we made evident the diversity inside the collection and the proximity between the Peruvian and the African groups. Using molecular techniques such as the microsatellites is important as they are supplementary to that of the descriptors. Besides, they play an essential role in the assessment of promising crops in countries like Peru.
|Date of Award||14 Sep 2009|
|Supervisor||Pierre Van Cutsem (Supervisor), Johan MESSIAEN (President), Nicolas Dauchot (Jury), Olivier Hardy (Jury) & Pierre BERTIN (Jury)|