Abstract
The frequency of transposition of Tnlac to pGC200, an IncFII R plasmid, increased during the storage of the host strain. This result is explained by the fact that the transpositional event is temperature-dependent: it occurred readily when the host strain was grown at 30 degrees C but it was nearly undetectable when the host strain was grown and kept at 37 degrees C. Fusions between two different plasmids carrying Tnlac with pGC200 were also thermosensitive, suggesting a relation between cointegrate formation and transposition. Lactose did not influence the frequency of transposition of Tnlac.
Original language | English |
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Pages (from-to) | 243-7 |
Number of pages | 5 |
Journal | Journal of general microbiology |
Volume | 117 |
Issue number | 1 |
Publication status | Published - 1980 |