Transposition of Tn951 (Tnlac) and cointegrate formation are thermosensitive processes

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Abstract

The frequency of transposition of Tnlac to pGC200, an IncFII R plasmid, increased during the storage of the host strain. This result is explained by the fact that the transpositional event is temperature-dependent: it occurred readily when the host strain was grown at 30 degrees C but it was nearly undetectable when the host strain was grown and kept at 37 degrees C. Fusions between two different plasmids carrying Tnlac with pGC200 were also thermosensitive, suggesting a relation between cointegrate formation and transposition. Lactose did not influence the frequency of transposition of Tnlac.
Original languageEnglish
Pages (from-to)243-7
Number of pages5
JournalJournal of general microbiology
Volume117
Issue number1
Publication statusPublished - 1980

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