The Tn3-family of Replicative Transposons

Emilien Nicolas, Michael Lambin, Damien Dandoy, Christine Galloy, Nathan Nguyen, Cédric A Oger, Bernard Hallet

Research output: Contribution to journalReview article

Abstract

Transposons of the Tn3 family form a widespread and remarkably homogeneous group of bacterial transposable elements in terms of transposition functions and an extremely versatile system for mediating gene reassortment and genomic plasticity owing to their modular organization. They have made major contributions to antimicrobial drug resistance dissemination or to endowing environmental bacteria with novel catabolic capacities. Here, we discuss the dynamic aspects inherent to the diversity and mosaic structure of Tn3-family transposons and their derivatives. We also provide an overview of current knowledge of the replicative transposition mechanism of the family, emphasizing most recent work aimed at understanding this mechanism at the biochemical level. Previous and recent data are put in perspective with those obtained for other transposable elements to build up a tentative model linking the activities of the Tn3-family transposase protein with the cellular process of DNA replication, suggesting new lines for further investigation. Finally, we summarize our current view of the DNA site-specific recombination mechanisms responsible for converting replicative transposition intermediates into final products, comparing paradigm systems using a serine recombinase with more recently characterized systems that use a tyrosine recombinase.

Original languageEnglish
JournalMicrobiology spectrum
Volume3
Issue number4
DOIs
Publication statusPublished - 2015

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Recombinases
DNA Transposable Elements
Microbial Drug Resistance
DNA Replication
Serine
Genetic Recombination
Tyrosine
Bacteria
DNA
Genes
Proteins
Tn3 transposase

Keywords

  • Bacteria/enzymology
  • Bacterial Proteins/genetics
  • DNA Transposable Elements
  • Recombination, Genetic
  • Transposases/genetics

Cite this

Nicolas, Emilien ; Lambin, Michael ; Dandoy, Damien ; Galloy, Christine ; Nguyen, Nathan ; Oger, Cédric A ; Hallet, Bernard. / The Tn3-family of Replicative Transposons. In: Microbiology spectrum. 2015 ; Vol. 3, No. 4.
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The Tn3-family of Replicative Transposons. / Nicolas, Emilien; Lambin, Michael; Dandoy, Damien; Galloy, Christine; Nguyen, Nathan; Oger, Cédric A; Hallet, Bernard.

In: Microbiology spectrum, Vol. 3, No. 4, 2015.

Research output: Contribution to journalReview article

TY - JOUR

T1 - The Tn3-family of Replicative Transposons

AU - Nicolas, Emilien

AU - Lambin, Michael

AU - Dandoy, Damien

AU - Galloy, Christine

AU - Nguyen, Nathan

AU - Oger, Cédric A

AU - Hallet, Bernard

PY - 2015

Y1 - 2015

N2 - Transposons of the Tn3 family form a widespread and remarkably homogeneous group of bacterial transposable elements in terms of transposition functions and an extremely versatile system for mediating gene reassortment and genomic plasticity owing to their modular organization. They have made major contributions to antimicrobial drug resistance dissemination or to endowing environmental bacteria with novel catabolic capacities. Here, we discuss the dynamic aspects inherent to the diversity and mosaic structure of Tn3-family transposons and their derivatives. We also provide an overview of current knowledge of the replicative transposition mechanism of the family, emphasizing most recent work aimed at understanding this mechanism at the biochemical level. Previous and recent data are put in perspective with those obtained for other transposable elements to build up a tentative model linking the activities of the Tn3-family transposase protein with the cellular process of DNA replication, suggesting new lines for further investigation. Finally, we summarize our current view of the DNA site-specific recombination mechanisms responsible for converting replicative transposition intermediates into final products, comparing paradigm systems using a serine recombinase with more recently characterized systems that use a tyrosine recombinase.

AB - Transposons of the Tn3 family form a widespread and remarkably homogeneous group of bacterial transposable elements in terms of transposition functions and an extremely versatile system for mediating gene reassortment and genomic plasticity owing to their modular organization. They have made major contributions to antimicrobial drug resistance dissemination or to endowing environmental bacteria with novel catabolic capacities. Here, we discuss the dynamic aspects inherent to the diversity and mosaic structure of Tn3-family transposons and their derivatives. We also provide an overview of current knowledge of the replicative transposition mechanism of the family, emphasizing most recent work aimed at understanding this mechanism at the biochemical level. Previous and recent data are put in perspective with those obtained for other transposable elements to build up a tentative model linking the activities of the Tn3-family transposase protein with the cellular process of DNA replication, suggesting new lines for further investigation. Finally, we summarize our current view of the DNA site-specific recombination mechanisms responsible for converting replicative transposition intermediates into final products, comparing paradigm systems using a serine recombinase with more recently characterized systems that use a tyrosine recombinase.

KW - Bacteria/enzymology

KW - Bacterial Proteins/genetics

KW - DNA Transposable Elements

KW - Recombination, Genetic

KW - Transposases/genetics

U2 - 10.1128/microbiolspec.MDNA3-0060-2014

DO - 10.1128/microbiolspec.MDNA3-0060-2014

M3 - Review article

C2 - 26350313

VL - 3

JO - Microbiology spectrum

JF - Microbiology spectrum

SN - 2165-0497

IS - 4

ER -