Sensitive assay for nicotinamide adenine dinucleotide phosphate and its reduced form based on the bioluminescence method

José Remacle, Andrée Houbion, Martine Raes

Research output: Contribution to journalArticlepeer-review

Abstract

An assay of reduced nicotinamide adenine dinucleotide phosphate (NADPH) by bioluminescence was investigated and applied for NADP+. The NADP+ is first reduced by glucose-6-phosphate dehydrogenase and then assayed in a mixture containing a NADPH/flavin mononucleotide oxidoreductase which in turn activates luciferase. Many interferences were observed and the method was modified accordingly. NADP+ and NADPH can be assayed separately or simultaneously within the range 1-100 pmol, which is sufficiently sensitive to be applied to biological materials. Many details and precautions must be taken into consideration.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalAnalytica chimica acta
Volume170
Issue numberC
DOIs
Publication statusPublished - 1985

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