Sensitive assay for nicotinamide adenine dinucleotide phosphate and its reduced form based on the bioluminescence method

José Remacle; Andrée Houbion; Martine Raes

doi:10.1016/S0003-2670(00)81732-8

Sensitive assay for nicotinamide adenine dinucleotide phosphate and its reduced form based on the bioluminescence method

José Remacle, Andrée Houbion, Martine Raes

Laboratory of Cellular Biochemistry and Biology

Research output: Contribution to journal › Article › peer-review

Abstract

An assay of reduced nicotinamide adenine dinucleotide phosphate (NADPH) by bioluminescence was investigated and applied for NADP⁺. The NADP⁺ is first reduced by glucose-6-phosphate dehydrogenase and then assayed in a mixture containing a NADPH/flavin mononucleotide oxidoreductase which in turn activates luciferase. Many interferences were observed and the method was modified accordingly. NADP⁺ and NADPH can be assayed separately or simultaneously within the range 1-100 pmol, which is sufficiently sensitive to be applied to biological materials. Many details and precautions must be taken into consideration.

Original language	English
Pages (from-to)	109-116
Number of pages	8
Journal	Analytica chimica acta
Volume	170
Issue number	C
DOIs	https://doi.org/10.1016/S0003-2670(00)81732-8
Publication status	Published - 1985

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10.1016/S0003-2670(00)81732-8

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title = "Sensitive assay for nicotinamide adenine dinucleotide phosphate and its reduced form based on the bioluminescence method",

abstract = "An assay of reduced nicotinamide adenine dinucleotide phosphate (NADPH) by bioluminescence was investigated and applied for NADP+. The NADP+ is first reduced by glucose-6-phosphate dehydrogenase and then assayed in a mixture containing a NADPH/flavin mononucleotide oxidoreductase which in turn activates luciferase. Many interferences were observed and the method was modified accordingly. NADP+ and NADPH can be assayed separately or simultaneously within the range 1-100 pmol, which is sufficiently sensitive to be applied to biological materials. Many details and precautions must be taken into consideration.",

author = "Jos{\'e} Remacle and Andr{\'e}e Houbion and Martine Raes",

year = "1985",

doi = "10.1016/S0003-2670(00)81732-8",

language = "English",

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journal = "Analytica chimica acta",

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T1 - Sensitive assay for nicotinamide adenine dinucleotide phosphate and its reduced form based on the bioluminescence method

AU - Remacle, José

AU - Houbion, Andrée

AU - Raes, Martine

PY - 1985

Y1 - 1985

N2 - An assay of reduced nicotinamide adenine dinucleotide phosphate (NADPH) by bioluminescence was investigated and applied for NADP+. The NADP+ is first reduced by glucose-6-phosphate dehydrogenase and then assayed in a mixture containing a NADPH/flavin mononucleotide oxidoreductase which in turn activates luciferase. Many interferences were observed and the method was modified accordingly. NADP+ and NADPH can be assayed separately or simultaneously within the range 1-100 pmol, which is sufficiently sensitive to be applied to biological materials. Many details and precautions must be taken into consideration.

AB - An assay of reduced nicotinamide adenine dinucleotide phosphate (NADPH) by bioluminescence was investigated and applied for NADP+. The NADP+ is first reduced by glucose-6-phosphate dehydrogenase and then assayed in a mixture containing a NADPH/flavin mononucleotide oxidoreductase which in turn activates luciferase. Many interferences were observed and the method was modified accordingly. NADP+ and NADPH can be assayed separately or simultaneously within the range 1-100 pmol, which is sufficiently sensitive to be applied to biological materials. Many details and precautions must be taken into consideration.

UR - http://www.scopus.com/inward/record.url?scp=21444452613&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(00)81732-8

DO - 10.1016/S0003-2670(00)81732-8

M3 - Article

AN - SCOPUS:21444452613

SN - 0003-2670

VL - 170

SP - 109

EP - 116

JO - Analytica chimica acta

JF - Analytica chimica acta

IS - C

ER -

Sensitive assay for nicotinamide adenine dinucleotide phosphate and its reduced form based on the bioluminescence method

Abstract

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