TY - JOUR
T1 - Psidium guajava L.- dichloromethane and ethyl acetate fractions ameliorate striped catfish (Pangasianodon hypophthalmus) status via immune response, inflammatory, and apoptosis pathways
AU - Nhu, Truong Quynh
AU - Hang, Bui Thi Bich
AU - Huong, Do Thi Thanh
AU - Scippo, Marie Louise
AU - Phuong, Nguyen Thanh
AU - Quetin-Leclercq, Joëlle
AU - Kestemont, Patrick
N1 - Funding Information:
The experiments were supported by the Commission of Cooperation and Development of the Académie de Recherche et d’Enseignement Supérieur (ARES-CCD) and the General Directorate for Cooperation and Development (DGD) in Belgium through the AquaBioActive Research Project for Development between the University of Namur , the University of Liège and the Université Catholique de Louvain in Belgium , and the Can Tho University in Vietnam .
Funding Information:
The experiments were supported by the Commission of Cooperation and Development of the Académie de Recherche et d'Enseignement Supérieur (ARES-CCD) and the General Directorate for Cooperation and Development (DGD) in Belgium through the AquaBioActive Research Project for Development between the University of Namur, the University of Liège and the Université Catholique de Louvain in Belgium, and the Can Tho University in Vietnam. The authors thank Prof. Bui Thi Buu Hue and Dr. Le Thi Bach for the supply of the plant fractions used in this study. We are very grateful to Dr. Carolin Mayer (ILEE scientific manager) for her revision of the English syntax.
Publisher Copyright:
© 2023
PY - 2023/7
Y1 - 2023/7
N2 - Psidium guajava L. is known to possess immune-modulatory properties in humans and other mammals. Although the positive effects of P. guajava-based diets on the immunological status have been shown for some fish species, the underlying molecular mechanisms of its protective effects remain to be investigated. The aims of this study were to evaluate the immune-modulatory effects of two guava fractions from dichloromethane (CC) and ethyl acetate (EA) on striped catfish with in vitro and in vivo experiments. Striped catfish head kidney leukocytes were stimulated with 40, 20, 10 and 0 μg/ml of each extract fraction, and the immune parameters (ROS, NOS, and lysozyme) were examined at 6 and 24 h post stimulation. A final concentration of each fraction at 40, 10 and 0 μg/fish was then intraperitoneally injected into the fish. After 6, 24, and 72 h of administration, immune parameters as well as the expression of some cytokines related to innate and adaptive immune responses, inflammation, and apoptosis were measured in the head kidney. Results indicated that the humoral (lysozyme) and cellular (ROS and NOS) immune endpoints were regulated differently by CC and EA fractions depending on dose and time in both, in vitro and in vivo experiments. With regards to the in vivo experiment, the CC fraction of the guava extract could significantly enhance the TLRs-MyD88-NF-κB signaling pathway by upregulating its cytokine genes (tlr1, tlr4, myd88, and traf6), following the upregulation of inflammatory (nfκb, tnf, il1β, and il6) and apoptosis (tp53 and casp8) genes 6 h after injection. Moreover, fish treated with both CC and EA fractions significantly enhanced cytokine gene expression including lys and inos at the later time points - 24 h or 72 h. Our observations suggest that P. guajava fractions modulate the immune, inflammatory, and apoptotic pathways.
AB - Psidium guajava L. is known to possess immune-modulatory properties in humans and other mammals. Although the positive effects of P. guajava-based diets on the immunological status have been shown for some fish species, the underlying molecular mechanisms of its protective effects remain to be investigated. The aims of this study were to evaluate the immune-modulatory effects of two guava fractions from dichloromethane (CC) and ethyl acetate (EA) on striped catfish with in vitro and in vivo experiments. Striped catfish head kidney leukocytes were stimulated with 40, 20, 10 and 0 μg/ml of each extract fraction, and the immune parameters (ROS, NOS, and lysozyme) were examined at 6 and 24 h post stimulation. A final concentration of each fraction at 40, 10 and 0 μg/fish was then intraperitoneally injected into the fish. After 6, 24, and 72 h of administration, immune parameters as well as the expression of some cytokines related to innate and adaptive immune responses, inflammation, and apoptosis were measured in the head kidney. Results indicated that the humoral (lysozyme) and cellular (ROS and NOS) immune endpoints were regulated differently by CC and EA fractions depending on dose and time in both, in vitro and in vivo experiments. With regards to the in vivo experiment, the CC fraction of the guava extract could significantly enhance the TLRs-MyD88-NF-κB signaling pathway by upregulating its cytokine genes (tlr1, tlr4, myd88, and traf6), following the upregulation of inflammatory (nfκb, tnf, il1β, and il6) and apoptosis (tp53 and casp8) genes 6 h after injection. Moreover, fish treated with both CC and EA fractions significantly enhanced cytokine gene expression including lys and inos at the later time points - 24 h or 72 h. Our observations suggest that P. guajava fractions modulate the immune, inflammatory, and apoptotic pathways.
KW - Cytokine genes
KW - Guava
KW - Head kidney leukocytes
KW - Lysozyme
KW - Nitric oxide synthase
KW - Respiratory burst activity
UR - http://www.scopus.com/inward/record.url?scp=85162778116&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2023.108851
DO - 10.1016/j.fsi.2023.108851
M3 - Article
C2 - 37245678
AN - SCOPUS:85162778116
SN - 1050-4648
VL - 138
JO - Fish & Shellfish Immunology
JF - Fish & Shellfish Immunology
M1 - 108851
ER -
