Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase

Bassam Al Balaa, Johan Wouters, Sophie Dogne, Carlos Rossini, Jean Michel Schaus, Eric Depiereux, Jean Vandenhaute, Isabelle Housen

Research output: Contribution to journalArticle

Abstract

We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZαA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56°C. The Michaelis-Menten constants were determined.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalBioscience, biotechnology and biochemistry
Volume70
Issue number1
DOIs
Publication statusPublished - 2 Feb 2006

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Keywords

  • Acidophilicity
  • Cloning
  • Fungus
  • Scytalidium acidophilum
  • Xylanase

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