Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase

Bassam Al Balaa; Johan Wouters; Sophie Dogne; Carlos Rossini; Jean Michel Schaus; Eric Depiereux; Jean Vandenhaute; Isabelle Housen

doi:10.1271/bbb.70.269

Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase

Bassam Al Balaa, Johan Wouters, Sophie Dogne, Carlos Rossini, Jean Michel Schaus, Eric Depiereux, Jean Vandenhaute, Isabelle Housen

Research output: Contribution to journal › Article › peer-review

Abstract

We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZαA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56°C. The Michaelis-Menten constants were determined.

Original language	English
Pages (from-to)	269-272
Number of pages	4
Journal	Bioscience, biotechnology and biochemistry
Volume	70
Issue number	1
DOIs	https://doi.org/10.1271/bbb.70.269
Publication status	Published - 2 Feb 2006

Keywords

Acidophilicity
Cloning
Fungus
Scytalidium acidophilum
Xylanase

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10.1271/bbb.70.269

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abstract = "We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZαA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56°C. The Michaelis-Menten constants were determined.",

keywords = "Acidophilicity, Cloning, Fungus, Scytalidium acidophilum, Xylanase",

author = "{Al Balaa}, Bassam and Johan Wouters and Sophie Dogne and Carlos Rossini and Schaus, {Jean Michel} and Eric Depiereux and Jean Vandenhaute and Isabelle Housen",

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T1 - Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase

AU - Al Balaa, Bassam

AU - Wouters, Johan

AU - Dogne, Sophie

AU - Rossini, Carlos

AU - Schaus, Jean Michel

AU - Depiereux, Eric

AU - Vandenhaute, Jean

AU - Housen, Isabelle

PY - 2006/2/2

Y1 - 2006/2/2

N2 - We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZαA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56°C. The Michaelis-Menten constants were determined.

AB - We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZαA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56°C. The Michaelis-Menten constants were determined.

KW - Acidophilicity

KW - Cloning

KW - Fungus

KW - Scytalidium acidophilum

KW - Xylanase

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SN - 0916-8451

VL - 70

SP - 269

EP - 272

JO - Bioscience, biotechnology and biochemistry

JF - Bioscience, biotechnology and biochemistry

IS - 1

ER -

Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase

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Keywords

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